Estrogen induction of progestin receptors in the hypothalamus of male and female rats which differ in their ability to exhibit cyclic gonadotropin secretion and female sexual behavior.
Estrogen induction of progestin receptors in the hypothalamus of male and female rats which differ in their ability to exhibit cyclic gonadotropin secretion and female sexual behavior.
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雌激素对雄性和雌性大鼠下丘脑中孕激素受体的诱导，其表现出周期性促性腺激素分泌和雌性性行为的能力不同。
DOI：
10.1095/biolreprod25.2.307
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发表时间：
1981
影响因子：
3.6
通讯作者：
A. M. Etgen
中科院分区：
文献类型：
--
作者：
A. M. Etgen
本研究考察了雌性和雄性大鼠下丘脑胞质黄体酮受体的雌激素诱导是否足以促进女性性行为和/或促性腺激素的分泌。新生雄性大鼠注射油(M)或在出生时阉割后注射油（D1-M）或10 μ g丙酸睾酮（TP） （10 -TP-M）；新生雌性小鼠分别用油(F)、10 Mg TP （10-TP-F）或500 g TP （500-TP-F）处理。成年后，通过阴道涂片检测女性的发情周期。在肾上腺腺切除术后，所有的动物都接受了雌激素和黄体酮的治疗，并对雌性性行为的接受性和感知性成分进行了测试。停用激素后，动物在献祭前24和48 h分别注射精油或5 g苯甲酸雌二醇（EB），行为学试验中使用相同剂量和次数的EB注射，并使用3H-R5020检测下丘脑-视前区（I-IPOA）细胞质孕激素受体的浓度。只有对照雌性(F)在成年后表现出阴道周期和黄体。F、10-TP-F、D1-M和10-TP-M组均表现出高水平的接受行为（前凸）；然而，只有F表现出高水平的感知行为（耳朵摆动、跳跃和飞奔）。无论是M组还是500-TP-F组都没有表现出明显的女性性行为。尽管新生处理大鼠对雌激素和黄体酮的反应能力与促性腺激素释放和雌性生殖行为存在很大差异，但EB处理在所有动物的HPOA中产生了相同的两倍的细胞质黄体酮受体水平增加。这些结果表明，雌激素诱导的神经黄体酮受体浓度升高不足以介导促性腺激素的排卵前激增，促进女性的性行为，也不足以解释这些反应对雌激素和黄体酮敏感性的男女差异。
This study examined whether estrogen induction of cytoplasmic progestin receptors in the hypothalamus of male and female rats is sufficient to facilitate feminine sexual behaviors and/or cyclic gonadotropin secretion. Newborn male rats were injected with oil (M) or were castrated at birth and injected with oil (D1-M) or 10 �g of testosterone propionate (TP) (l0-TP-M); neonatal females were treated with oil (F), 10 Mg of TP (10-TP-F), or 500 �g of TP (500-TP-F). In adulthood, females were tested for estrous cyclicity by taking vaginal smears. Following adrenalectomygonadectomy, all animals received estrogen plus progesterone and were tested for the display of receptive and proceptive components of female sexual behavior. After hormone withdrawal, animals were injected with oil or 5 �g of estradiol benzoate (EB) 24 and 48 h before sacrifice, the same dose and times of EB injection used in behavioral testing, and the concentrations of cytoplasmic progestin receptors in the hypothalarnus-preoptic area (I-IPOA) were determined using 3H-R5020. Only control females (F) exhibited vaginal cyclicity and corpora lutea as adults. F, 10-TP-F, D1-M, and 10-TP-M groups all showed high levels of receptive hehavior (lordosis); however, only F showed high levels of proceptive behavior (ear wiggling, hopping, and darting). Neither M nor 500-TP-F groups displayed significant levels of any female sexual behaviors. Despite these large differences in the capacity of neonatally manipulated rats to respond to estrogen and progesterone with gonadotropin release and female reproductive behaviors, EB treatment produced identical, twofold increases in cytoplasmic progestin receptor levels in the HPOA of all animals. These results demonstrate that estrogen-induced elevation of neural progestin receptor concentrations alone is not sufficient to mediate the preovulatory surge of gonadotropmns, to facilitate female sexual behavior, or to underlie male-female differences in the sensitivity of these responses to estrogen and progesterone.