Chapter 2 THE DFRC ( DERIVATIZATION FOLLOWED BY REDUCTIVE CLEAVAGE ) METHOD AND ITS APPLICATIONS FOR LIGNIN CHARACTERIZATION
Chapter 2 THE DFRC ( DERIVATIZATION FOLLOWED BY REDUCTIVE CLEAVAGE ) METHOD AND ITS APPLICATIONS FOR LIGNIN CHARACTERIZATION
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第2章 DFRC（还原裂解衍生化）方法及其在木质素表征中的应用
DOI：
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
J. Ralph
中科院分区：
文献类型：
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作者：
F. Lu;J. Ralph
木质素可能是陆生植物中存在的最复杂的天然聚合物。由于木质素的重要性（在各个领域）和复杂性（组成和结构），木质素的表征是重要且具有挑战性的。DFRC方法是作为木质素表征的分析方法开发的，不同于现有的水解方法。它通过选择性裂解木质素中最重要的β-O-4-键，产生单体和二聚体，以便通过GC或GC-MS进行分析。木质素的（组成和结构）测定，包括木质素生物合成领域的结构鉴定，描绘木质纤维素中的生物化学过程（包括在各种突变体和转基因植物系中），以及在食用植物研究中。一个独特的特征是木质素γ-酯（天然存在于许多植物物种中）在该方案期间不裂解，使得可以检测天然木质素酰化。DFRC方法的修改版本甚至已用于检测木质素上天然存在的乙酸酯。对红麻木质素中非树脂醇四氢呋喃结构衍生的带有γ-乙酸酯的独特β-β-偶联DFRC二聚体的检测和鉴定证实，乙酰化木质素是由预乙酰化单木质素（主要是γ-乙酸芥子酯）的参与产生的。此外，结合其他分析工具，DFRC分析提供了有关木质素结构和木质素生物合成途径的信息数据。
Lignin is perhaps the most complex natural polymer present in terrestrial plants. Characterization of lignin is important and challenging because of the significance (in various fields) and complexity (compositional and structural) of lignin. The DFRC method was developed as an analytical procedure for lignin characterization that differed from the existing hydrolytic methods. It operates via selective cleavage of the most important β–O–4-linkages in lignin, producing monomers and dimers for ready analysis by GC or GC-MS. It has been used for the characterization (compositional and structural) determination of lignins, including for structural identification in the area of lignin biosynthesis, delineating biochemical processes in lignocellulosics (including in various mutant and transgenic plant lines), and in food plant research. One unique feature is that lignin γ-esters (naturally occurring in many plant species) are not cleaved during the protocol so that natural lignin acylation can be detected. A modified version of the DFRC method has even been used to detect naturally occurring acetates on lignin. Detection and identification of the unique β–β-coupled DFRC dimers bearing γ-acetates that derive from non-resinol tetrahydrofuran structures in kenaf lignin confirmed that acetylated lignins result from the participation of the pre-acetylated monolignols, primarily sinapyl γ-acetate. Furthermore, in combination with other analytical tools, DFRC analysis has provided informative data about lignin structures and the lignin biosynthetic pathway.
影响因子：
3.8
作者：
Ralph, J;Lapierre, C;Boerjan, W
通讯作者：
Boerjan, W