The Cryoprotective Effects of Vitamin B12 Supplementation on Bovine Semen Quality
The Cryoprotective Effects of Vitamin B12 Supplementation on Bovine Semen Quality
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DOI：
10.1111/j.1439-0531.2009.01575.x
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发表时间：
2011-02-01
影响因子：
1.7
通讯作者：
Li, Q-W
中科院分区：
文献类型：
--
作者：
Hu, J-H;Tian, W-Q;Li, Q-W
本研究旨在探讨添加维生素B-12对牛精液质量指标和抗氧化酶活性的影响。在牛精液冷冻保护液中分别添加1.25、2.5、3.75和5.0 mg/ml的维生素B-12。结果表明，添加2.50 mg/ml维生素B-12的精子活力、直线速度、曲线速度、平均系数、平均路径速度均显著高于其他组(p<；0.05)。在含有2.50和3.75 mg/ml维生素B-12的稀释液中，线性指数、头部侧位值和A级精子百分率没有显著差异(p>；0.05)。添加2.50 mg/ml维生素B-12可显著提高顶体完整率和质膜完整率(p<；0.05)。生化检测结果显示，补充维生素B-12组与对照组相比，超氧化物歧化酶水平无明显变化(p>；0.05)。然而，添加2.50 mg/ml维生素B-12组的过氧化氢酶水平高于其他组(p<；0.05)。添加维生素B-12的填充剂显著降低了谷胱甘肽过氧化物酶活性(p<；0.05)。与其他组相比，添加3.75 mg/ml维生素B-12组的谷胱甘肽还原酶活性最高(p<；0.05)。综上所述，添加维生素B-12的牛精液稀释液可以减轻冷冻解冻引起的氧化应激，改善牛精液品质。关于冷冻保存的牛精液中维生素B-12的脂质过氧化和抗氧化能力的测定，还需要进一步的研究才能获得更具体的结果。
ContentsThe present study aimed to investigate the effects of vitamin B-12 supplementation on standard bovine semen quality parameters and anti-oxidative enzyme activities. Vitamin B-12 was supplemented at concentrations of 1.25, 2.5, 3.75 and 5.0 mg/ml to bovine semen cryoprotective medium. The results indicated that the motility and straight line velocity, curvilinear velocity, mean coefficient, velocity of the average path values of sperm supplemented with 2.50 mg/ml vitamin B-12 were significantly higher than that of other groups (p < 0.05). No significant difference was observed for linearity index, lateral head displacement values and the percentage of grade A spermatozoa between the extenders containing 2.50 and 3.75 mg/ml vitamin B-12 (p > 0.05). The percentages of acrosome-intact and plasma membrane-intact spermatozoa were significantly improved (p < 0.05) by supplementing with 2.50 mg/ml vitamin B-12. The results of biochemical assay revealed that vitamin B-12 supplementation did not cause significant changes in superoxide dismutase levels compared with control (p > 0.05). However, the catalase levels were higher in the treatment supplemented with vitamin B-12 at 2.50 mg/ml, when compared with other groups (p < 0.05). The extender supplemented with vitamin B-12 significantly decreased glutathione peroxidase activity compared with the control (p < 0.05). The supplementation of 3.75 mg/ml vitamin B-12 caused the highest value of glutathione reductase activity, compared with other groups (p < 0.05). In conclusion, the extender supplemented with vitamin B-12 could reduce the oxidative stress provoked by freezing-thawing and improve bovine semen quality. Further studies are required to obtain more concrete results on the determination of lipid peroxidation and antioxidant capacities of vitamin B-12 in cryopreserved bovine semen.