Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir
Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir
复制标题
通过给小鼠注射 shZEB1 质粒或 miR200c agomir 增强 B16F10/GPI-IL-21 疫苗对黑色素瘤的功效
DOI：
10.1016/j.biopha.2016.03.013
复制
发表时间：
2016-05-01
影响因子：
7.5
通讯作者：
Dou, Jun
中科院分区：
文献类型：
--
作者：
Wang, Xiaoying;Zhao, Fengshu;Dou, Jun
In this study, we hypothesized that the inhibition of epithelial to mesenchymal transition (EMT) program by knockdown of Zinc-finger E-box binding homeobox 1 (ZEB1) or administration of miR200c agomir would strengthen the B16F10 cells transfected with GPI-anchored IL-21 (B16F10/GPI-IL-21) vaccine efficacy in inhibiting the melanoma metastasis. Our findings from the current study indicated that, when compared with the mice immunized with the B16F10/GPI-IL-21 vaccine alone, the mice immunized with B16F10/GPI-IL-21 vaccine combined with injection of shZEB1 plasmid or miR200c agomir not only meaningfully inhibited EMT of melanoma, reduced the EMT characteristic molecular expression in tumor tissues, but also significantly decreased the Treg cells and TGF-beta 1, enhanced the cytotoxicities of NK cells and cytotoxic T lymphocytes and the IFN-gamma level. Furthermore, the immunotherapeutic combination resulted in inhibiting the melanoma growth and lung metastasis. Our study demonstrated that using the B16F10/GPI-IL-21 vaccine in combination with the down-regulated ZEB1 or miR200c administration effectively elicited anti-tumor immunity and reduced melanoma metastasis by inhibiting the EMT program in the B16F10 melanoma-bearing mice. (C) 2016 Elsevier Masson SAS. All rights reserved.
