Large-Area Aligned Fullerene Nanocrystal Scaffolds as Culture Substrates for Enhancing Mesenchymal Stem Cell Self-Renewal and Multipotency
Large-Area Aligned Fullerene Nanocrystal Scaffolds as Culture Substrates for Enhancing Mesenchymal Stem Cell Self-Renewal and Multipotency
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DOI：
10.1021/acsanm.0c00973
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发表时间：
2020-07-24
影响因子：
5.9
通讯作者：
Ariga，Katsuhiko
中科院分区：
文献类型：
--
作者：
Song，Jingwen;Jia，Xiaofang;Ariga，Katsuhiko
基于人间充质干细胞（hMSC）的疗法的发展受到成体干细胞的有限可用性的阻碍。多能性和干细胞样表型的长期保留仍然是大规模体外扩增过程中的关键挑战。在本文中，我们报告了使用高度对齐的富勒烯纳米晶须纳米图案支架来精确调节hMSCs的粘附和功能的主要例子。采用一种简单的Langmuir-Blodgett方法实现了富勒烯纳米晶须的连续可调排列。我们已经发现，在高度对齐的富勒烯纳米晶须纳米图案化表面上培养的细胞中，通过适当的细胞收缩性和Yes相关蛋白的核定位，长期多能性保留和再生能力得到增强。这项研究强调了对齐的富勒烯纳米晶须纳米图案化表面作为干细胞体外扩增和组织工程的有吸引力的平台的重要性。
Development of human mesenchymal stem cell (hMSC)-based therapies is hindered by the limited availability of adult stem cells. Long-term retention of multipotency and stem-like phenotypes remains a key challenge in the large-scalein vitroexpansion process. In this paper, we report the primary example of the use of highly aligned fullerene nanowhisker nanopatterned scaffolds to regulate precisely adhesion and function of hMSCs. Continuously tunable alignment of fullerene nanowhiskers was attained using a simple Langmuir–Blodgett approach. We have found that long-term multipotency retention and regenerative capacity are enhanced in cells cultured on the highly aligned fullerene nanowhisker nanopatterned surfaces mediated by the appropriate cell contractility and nucleus localization of Yes-associated protein. This study highlights the importance of the aligned fullerene nanowhisker nanopatterned surface as an attractive platform for stem cell expansionin vitroand for tissue engineering.