Picking winners in cell-cell collisions: Wetting, speed, and contact
Picking winners in cell-cell collisions: Wetting, speed, and contact
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DOI：
10.1103/physreve.106.054413
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发表时间：
2022-11-30
影响因子：
2.4
通讯作者：
Camley，Brian A.
中科院分区：
文献类型：
--
作者：
Zadeh，Pedrom;Camley，Brian A.
真核细胞群可以协调它们的爬行运动，以更有效地遵循线索、呆在一起或入侵新区域。这种集体细胞迁移取决于细胞与细胞之间的相互作用，这通常是通过将成对的细胞碰撞在一起来研究的。这些碰撞的结果可以预测吗？最近对碰撞上皮细胞序列的实验表明，与表面接触角较小或速度较大的细胞更有可能在碰撞时保持其方向（“获胜”）。我们什么时候应该期望形状或速度与碰撞结果相关？为了研究这个问题，我们在相场框架内建立了两个细胞碰撞的模型，该模型允许细胞形状变化。我们可以通过关于细胞如何相互作用的两种不同假设来再现具有高速和小接触角的细胞更有可能获胜的观察结果：（1）速度对齐，其中我们假设细胞感知自己的速度并在有限的时间尺度内与其对齐，以及（2）前前接触复极化，其中细胞极化远离细胞与细胞的接触，类似于运动的接触抑制。令人惊讶的是，尽管我们模拟了具有广泛不同属性的细胞之间的碰撞，但在每种情况下，细胞获胜的概率完全由单个汇总变量捕获：其相对速度（在速度对准模型中）或其相对接触角（在接触复极化模型中）。这两种模型目前与报道的实验结果一致，但可以通过正交扰动改变细胞接触角和速度来区分它们。
Groups of eukaryotic cells can coordinate their crawling motion to follow cues more effectively, stay together, or invade new areas. This collective cell migration depends on cell-cell interactions, which are often studied by colliding pairs of cells together. Can the outcome of these collisions be predicted? Recent experiments on trains of colliding epithelial cells suggest that cells with a smaller contact angle to the surface or larger speeds are more likely to maintain their direction (“win”) upon collision. When should we expect shape or speed to correlate with the outcome of a collision? To investigate this question, we build a model for two-cell collisions within the phase field framework, which allows for cell shape changes. We can reproduce the observation that cells with high speed and small contact angles are more likely to win with two different assumptions for how cells interact: (1) velocity aligning, in which we hypothesize that cells sense their own velocity and align to it over a finite timescale, and (2) front-front contact repolarization, where cells polarize away from cell-cell contact, akin to contact inhibition of locomotion. Surprisingly, though we simulate collisions between cells with widely varying properties, in each case, the probability of a cell winning is completely captured by a single summary variable: its relative speed (in the velocity-aligning model) or its relative contact angle (in the contact repolarization model). Both models are currently consistent with reported experimental results, but they can be distinguished by varying cell contact angle and speed through orthogonal perturbations.