Effects of soluble metals on human peri-implant cells
Effects of soluble metals on human peri-implant cells
复制标题
DOI：
10.1002/jbm.a.30345
复制
发表时间：
2005-07-01
影响因子：
4.9
通讯作者：
Jacobs, JJ
中科院分区：
文献类型：
--
作者：
Hallab, NJ;Anderson, S;Jacobs, JJ
尽管有报道称组织坏死与植入失败有关，但金属毒性等过程对植入性能产生负面影响的程度尚不清楚。我们评估了代表性的人类种植体周围细胞（即成骨细胞、成纤维细胞和淋巴细胞）受到各种浓度（0.01-10.0 mM）的 Al+3、Co+2' Cr+3、Fe+3' Mo+5、Ni+2 和 V+3 氯化物溶液（以及 Na+2 作为对照）的攻击。使用增殖测定、活力测定和显微细胞形态评估来测量细胞反应。研究发现，差异效应与其说是细胞类型的函数，不如说是金属挑战物的成分和浓度的函数。没有证明优先免疫抑制。低于 0.01 mM，没有金属是有毒的。在攻击浓度 < 1 mM 时，毒性最强的金属（即 Co、Ni 和 V）可将成骨细胞、成纤维细胞和淋巴细胞的增殖 (IC50)、活力 (LC50) 和细胞形态降低 < 50%。测试的所有其他金属需要 > 5 mM 才能获得相同的响应。在1 mM以下，这些有毒金属还诱导所有细胞形态的改变，包括丝状伪足或片状伪足的丧失或细胞形状的变化。在临床相关浓度（低于种植体周围组织/液体中先前报告的值）下有毒的金属包括对于淋巴细胞的 Co (0.6 mM)、Ni (0.8 mM)、V (0.5 mM)，以及对于成纤维细胞的 Co (0.8 mM)、V (0-3 mM)、Al (1-5 mM)、Fe (1-5 mM) 和 Co (0.8 mM)， Ni (0.7 mM)、V (0.1 mM) 用于成骨细胞。只有 Co 和 V 在体外浓度低于体内滑液中检测到的浓度时才具有毒性（对于成纤维细胞，V 为 0.1 mM，Co 为 0.8 mM；对于成骨细胞，V 为 0.4 mM，Co 为 0.8 mM）。因此，钴基合金和钛基合金分别释放的可溶性钴和钒可能最有可能介导假体周围环境中的细胞毒性。 (c) 2005 Wiley periodicals, Inc. J Biomed Mater Res 74A: 124-140, 2005。
Despite reports associating tissue necrosis with implant failure, the degree to which processes, such as metal toxicity, negatively impact implant performance is unknown. We evaluated representative human peri-implant cells (i.e., osteoblasts, fibroblasts, and lymphocytes) when challenged by Al+3, Co+2' Cr+3, Fe+3' Mo+5, Ni+2, and V+3 chloride solutions (and Na+2 as a control) over a wide range of concentrations (0.01-10.0 mM). Cell responses were measured using proliferation assays, viability assays, and microscopic cell morphology assessments. Differential effects were found to be less a function of the cell type than of the composition and concentration of metal challenge. No preferential immunosuppression was demonstrated. Below 0.01 mM, no metal was toxic. The most toxic metals (i.e., Co, Ni, and V) reduced proliferation (IC50), and viability (LC50) and cell morphology of osteoblasts, fibroblasts, and lymphocytes by < 50% at challenge concentrations < 1 mM. All other metals tested required > 5 mM to exact the same responses. Below I mM, these toxic metals also induced alterations in all cell morphology consisting of loss of filopodia or lamel-lipodia or changes in cell shape. Metals that were toxic at clinically relevant concentrations (less than previously reported values in peri-implant tissues/fluids) include Co (0.6 mM), Ni (0.8 mM), V (0.5 mM) for lymphocytes and Co (0.8 mM), V (0-3 mM), Al (1-5 mM), Fe (1-5 mM) for fibroblasts, and Co (0.8 mM), Ni (0.7 mM), V (0.1 mM) for osteoblasts. Only Co and V were toxic in vitro at concentrations below that detected in vivo in synovial fluid (V at 0.1 mM and Co at 0.8 mM for fibroblasts, and V at 0.4 mM and Co at 0.8 mM on osteoblasts). Thus, soluble Co and V released from Co- and Ti-based alloys, respectively, could be implicated as the most likely to mediate cell toxicity in the periprosthetic milieu. (c) 2005 Wiley Periodicals, Inc. J Biomed Mater Res 74A: 124-140, 2005.