Microscopic dissection of the process of stress granule assembly
Microscopic dissection of the process of stress granule assembly
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DOI：
10.1016/j.bbamcr.2009.08.010
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发表时间：
2009-11-01
影响因子：
5.1
通讯作者：
Murata, Masayuki
中科院分区：
文献类型：
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作者：
Fujimura, Ken;Katahira, Jun;Murata, Masayuki
应激颗粒（Stress granules，SG）是一种在细胞应激反应中形成的mRNA分类位点。为了研究SGs如何带来大规模的空间区室化，我们监测了各种RNA结合蛋白（RBP）在暴露于应激时靶向SGs的定位。我们发现，伴随着eIF2 α磷酸化的发生，RBP在细胞质中局部积累，这导致分子间相互作用增加，并形成强烈的洗涤剂抗性灶。随后，微管（MT）介导1）SG的有序空间组织和2）一组核定位的SG组分向细胞质的募集。与此同时，似乎不需要MT在SG分配组装后进行维护。我们的数据表明，SG的形成过程是由MT的非依赖性和依赖性途径，在应激反应中依次发生。(C)2009爱思唯尔有限公司版权所有。
Stress granules (SGs) are mRNA triage sites that are formed in response to a variety of cellular stress. To study how SGs bring about the massive spatial compartmentalization, we monitored the localization of various RNA-binding proteins (RBPs) targeted to SGs upon exposure to stress. We discovered that concomitant with the onset of eIF2 alpha phosphorylation, RBPs accumulate locally in the cytoplasm, which leads to increased inter-molecular interactions and the formation of robustly detergent-resistant foci. Subsequently, microtubules (MTs) mediate 1) the ordered spatial organization of SGs and 2) the recruitment of a set of nuclear-localized SG components to the cytoplasm. Meanwhile, MTs did not appear to be required for the maintenance of SG distribution after its assembly. Our data suggest that the process of SG formation is composed of MT-independent and -dependent pathways, which take place sequentially during stress response. (C) 2009 Elsevier B.V. All rights reserved.