Association of HLA-B39 with HLA-B27-negative ankylosing spondylitis and pauciarticular juvenile rheumatoid arthritis in Japanese patients. Evidence for a role of the peptide-anchoring B pocket.
Association of HLA-B39 with HLA-B27-negative ankylosing spondylitis and pauciarticular juvenile rheumatoid arthritis in Japanese patients. Evidence for a role of the peptide-anchoring B pocket.
复制标题
日本患者 HLA-B39 与 HLA-B27 阴性强直性脊柱炎和少关节型幼年类风湿关节炎的关联。
作者：
Akihiro Yamaguchi;N. Tsuchiya;H. Mitsui;M. Shiota;A. Ogawa;K. Tokunaga;S. Yoshinoya;T. Juji;Koji Ito
目的
探讨HLA-B27与血清阴性脊柱关节病易感性的关系。
方法
对日本强直性脊柱炎（AS）、幼年类风湿性关节炎（JRA）患者和健康对照者进行血清学HLA I类分型。通过聚合酶链反应-序列特异性寡杂交确定HLA-B39亚型。
结果
48例AS患者中有40例（83%）存在HLA-B27，而210例健康对照中仅1例（0.5%）存在HLA-B27。8例HLA-B27阴性的患者中有3例（37.5%）HLA-B39阳性，显著高于HLA-B27阴性对照组（6.2%; P = 0.01）。与HLA-B39的相关性在JRA患者中为16.7%（P < 0.01），尤其在少关节型患者中为33.3%（P < 0.01）。13例HLA-B39阳性患者中10例为B*3901亚型，3例为B*3902亚型。
结论
由于HLA-B27和HLA-B39共享45位的Glu和67位的Cys，这两者构成了肽锚定B口袋的组成部分，并且由于它们具有相似的肽配体基序，因此我们的结果可能支持I类抗原呈递的肽的作用或67位Cys在脊椎关节病和少关节型JRA的发展中的重要性。
OBJECTIVE
To investigate the characteristics of HLA-B27 that render susceptibility to seronegative spondylarthropathies.
METHODS
Serologic HLA class I typing of Japanese patients with ankylosing spondylitis (AS), juvenile rheumatoid arthritis (JRA), and healthy controls, was performed. HLA-B39 subtypes were determined by polymerase chain reaction-sequence-specific oligohybridization.
RESULTS
HLA-B27 was present in 40 of 48 patients with AS (83%), and in only 1 of 210 healthy controls (0.5%). Three of 8 patients (37.5%) who were negative for HLA-B27 were positive for HLA-B39, which was significantly higher compared with the HLA-B27-negative controls (6.2%; P = 0.01). Significant association with HLA-B39 was also noted in the JRA patients (16.7%; P < 0.01), especially in those patients with pauciarticular-onset disease (33.3%; P < 0.01). Ten of 13 HLA-B39-positive patients had subtype B*3901 and 3 had B*3902.
CONCLUSION
Because HLA-B27 and HLA-B39 share Glu at position 45 and Cys at position 67, both of which constitute components of the peptide-anchoring B pocket, and because they possess similar peptide-ligand motifs, our results may support either the role of the peptides presented by class I antigens or the importance of Cys at position 67, in the development of spondylarthropathies and pauciarticular-onset JRA.
影响因子：
32.4
作者：
R. Colbert;S. Rowland-Jones;A. McMichael;J. Frelinger
通讯作者：
R. Colbert;S. Rowland-Jones;A. McMichael;J. Frelinger
DOI：
10.1172/jci113708
发表时间：
1988-09
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
J. Taurog;F. El-Zaatari
通讯作者：
J. Taurog;F. El-Zaatari
通过质谱和计算机建模确定从 I 类 MHC 分子 HLA-B7 中洗脱的内源肽的特征。
DOI：
--
发表时间：
1993
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Huczko,EL;Bodnar,WM;Benjamin,D;Sakaguchi,K;Zhu,NZ;Shabanowitz,J;Henderson,RA;Appella,E;Hunt,DF;Engelhard,VH
通讯作者：
Engelhard,VH