Fc-dependent inhibition of mouse B cell activation by whole anti-mu antibodies.
Fc-dependent inhibition of mouse B cell activation by whole anti-mu antibodies.
复制标题
全抗 mu 抗体对小鼠 B 细胞活化的 Fc 依赖性抑制。
DOI：
10.4049/jimmunol.130.2.602
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发表时间：
1983
影响因子：
4.4
通讯作者：
D. Parker
中科院分区：
文献类型：
--
作者：
N. Phillips;D. Parker
我们一直使用全兔抗小鼠mu抗体来研究F（ab '）2抗mu抗体和抗原非特异性辅助因子（SN）对小鼠B细胞活化的Fc依赖性抑制。我们在这里表明，这种抑制似乎并不需要贴壁细胞，似乎发生独立的细胞相互作用，是可逆的，并不是由抑制因素单独维持。此外，兔抗体-抗原复合物对Fc受体的占据不足以抑制F（ab '）2抗mu和SN的激活。这些观察结果，连同通过蛋白A阻断兔抗mu抗体的Fc受体结合能力阻止抑制的观察结果，表明交联B细胞上的mlg和Fc γ受体阻止活化。然而，F（ab '）2抗mu和SN活化的B细胞在培养48小时后变得对这种抑制不敏感。
We have been using whole rabbit anti-mouse mu antibodies to study Fc-dependent inhibition of mouse B cell activation by F(ab')2 anti-mu antibodies and antigen-nonspecific helper factors (SN). We show here that this inhibition does not appear to require adherent cells, appears to occur independently of cellular interactions, is reversible, and is not maintained solely by suppressive factors. In addition, occupancy of Fc receptors by rabbit antibody-antigen complexes is not sufficient to inhibit activation by F(ab')2 anti-mu and SN. These observations, in conjunction with the observation that blocking the Fc receptor-binding capacity of rabbit anti-mu antibodies by protein A prevents inhibition, suggest that cross-linking mlg and Fcgamma receptors on B cells prevents activation. However, the F(ab')2 anti-mu and SN-activated B cells become refractory to this inhibition by 48 hr in culture.