Identification of Quantitative Trait Loci for Rice Quality in a Population of Chromosome Segment Substitution Lines
Identification of Quantitative Trait Loci for Rice Quality in a Population of Chromosome Segment Substitution Lines
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DOI：
10.1111/j.1744-7909.2009.00822.x
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发表时间：
2009-05-01
影响因子：
11.4
通讯作者：
Lin, Hong-Xuan
中科院分区：
文献类型：
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作者：
Hao, Wei;Zhu, Mei-Zhen;Lin, Hong-Xuan
对优质稻米的需求是稻米生产中的一个主要问题。稻米品质的主要组成部分包括外观、食用、烹饪、理化、碾磨和营养品质。这些性状大多是由数量性状位点（qtl）控制的复杂性状，因此与单基因控制的性状相比，这些性状的遗传鉴定更为困难。qtl的检测和遗传鉴定有助于深入了解品质性状的遗传机制。染色体片段代换系是定位qtl的有效工具。在本研究中，我们从‘Koshihikari’与Oryza sativa L. ssp杂交的回交后代（BC3F2）中构建了154个cssl。粳稻品种)作为循环亲本和‘Nona Bokra’(一个O. sativa L. ssp。籼稻品种)作为供体亲本。在此过程中，我们利用覆盖大部分水稻基因组的102个断裂扩增多态性序列和简单序列重复标记进行了标记辅助选择。最后，利用这组CSSLs进行水稻品质性状qtl的鉴定。检测到10个水稻外观品质性状qtl， 8个理化性状qtl。这些结果为进一步的遗传研究和改良籽粒品质的育种奠定了基础。
The demand for high quality rice represents a major issue in rice production. The primary components of rice grain quality include appearance, eating, cooking, physico-chemical, milling and nutritional qualities. Most of these traits are complex and controlled by quantitative trait loci (QTLs), so the genetic characterization of these traits is more difficult than that of traits controlled by a single gene. The detection and genetic identification of QTLs can provide insights into the genetic mechanisms underlying quality traits. Chromosome segment substitution lines (CSSLs) are effective tools used in mapping QTLs. In this study, we constructed 154 CSSLs from backcross progeny (BC3F2) derived from a cross between 'Koshihikari' (an Oryza sativa L. ssp. japonica variety) as the recurrent parent and 'Nona Bokra' (an O. sativa L. ssp. indica variety) as the donor parent. In this process, we carried out marker-assisted selection by using 102 cleaved amplified polymorphic sequence and simple sequence repeat markers covering most of the rice genome. Finally, this set of CSSLs was used to identify QTLs for rice quality traits. Ten QTLs for rice appearance quality traits were detected and eight QTLs concerned physico-chemical traits. These results supply the foundation for further genetic studies and breeding for the improvement of grain quality.