Elevated frequency of IL-37-and IL-18Rα-positive T cells in the peripheral blood of rheumatoid arthritis patients
Elevated frequency of IL-37-and IL-18Rα-positive T cells in the peripheral blood of rheumatoid arthritis patients
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DOI：
10.1016/j.cyto.2018.02.015
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发表时间：
2018-10-01
期刊：
影响因子：
3.8
通讯作者：
Lu, Jing
中科院分区：
文献类型：
--
作者：
Wang, Liuqing;Wang, Yueying;Lu, Jing
Objective: To detect the expression of IL-37 and its receptors IL-18Ra and IL-1R8 in CD4(+) T cells and total lymphocytes in rheumatoid arthritis (RA) patients and the relationship between autoantibodies and disease activity. To investigate the mechanism of IL-37 and its receptors involved in the pathogenesis of RA. To evaluate the effects of different concentrations of rhIL-37 on peripheral blood mononuclear cells (PBMCs) in RA patients with TNF-alpha, and IL-6.Methods: The expression of IL-37 and its receptor IL-18Ra and IL-1R8 in peripheral blood CD4(+) T cells and total lymphocytes in RA patients and healthy controls were measured by flow cytometry. The levels of TNF-alpha and IL-6 in the supernatant were measured by ELISA after rhIL-37 stimulation with PBMCs.Results: The expression of IL-37 and IL-18Ra in the total lymphocytes, especially in CD4(+) T cells in RA patients, was significantly higher than in the healthy control group. There was a positive correlation between the frequency of IL-37- or IL-18Ra-positive CD4(+) T cells and ESR, CRP, and DAS28 values. Additionally, rhlL-37 significantly down-regulated TNF-alpha and IL-6 production in RA patients' PBMCs.Conclusions: IL-37 plays an important role in the regulation of inflammation in RA. IL-37 and its receptors may play an immunoregulatory role in the activation of lymphocytes, especially CD4(+) T cells, in RA patients. IL-37 may represent a therapeutic target for rheumatoid arthritis.
