Antibacterial Surface Design of Titanium-Based Biomaterials for Enhanced Bacteria-Killing and Cell-Assisting Functions Against Periprosthetic Joint Infection
Antibacterial Surface Design of Titanium-Based Biomaterials for Enhanced Bacteria-Killing and Cell-Assisting Functions Against Periprosthetic Joint Infection
复制标题
钛基生物材料的抗菌表面设计，增强抗菌和细胞辅助功能，对抗假体周围感染
DOI：
10.1021/acsami.6b02803
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发表时间：
2016
影响因子：
9.5
通讯作者：
Chu Paul K.
中科院分区：
文献类型：
--
作者：
Wang Jiaxing;Li Jinhua;Qian Shi;Guo Geyong;Wang Qiaojie;Tang Jin;Shen Hao;Liu Xuanyong;Zhang Xianlong;Chu Paul K.
假体周围关节感染（PJI）是骨科手术后严重的并发症之一，抑制假体表面生物膜的形成是预防PJI的关键。然而，最近已经证明，局部体液中自由漂浮的生物膜样聚集体和植入物和植入物周围组织上的细菌定植可以共存，并参与PJI的发病机制。具有接触杀灭和释放杀灭抗微生物能力的有效表面可以潜在地减轻这些问题，并最大限度地减少由粘附/粘附细菌引起的PJI。本文中，Ag纳米颗粒（NPs）通过阳极氧化和等离子体浸没离子注入（PIII）嵌入二氧化钛（TiO 2）纳米管中以形成接触杀灭表面。然后通过真空提取和冻干将万古霉素掺入纳米管中以产生释放杀伤作用。建立了一种新的临床PJI模型系统，包括体外和体内使用甲氧西林耐药金黄色葡萄球菌（MRSA）ST 239，以系统地评估混合表面对嗜热菌和固着菌的抗菌性能。载万古霉素和银离子注入的二氧化钛纳米管表面表现出优异的抗菌和抗细菌/粘附细菌的抗菌膜效果，而没有明显的银离子释放。成纤维细胞/细菌共培养表明，表面可以帮助成纤维细胞对抗细菌。我们首先利用植入物表面的纳米结构作为无机抗菌剂（Ag NPs）和有机抗菌剂（万古霉素）之间的桥梁，在PJI的战斗中取得全面胜利。接触杀伤和释放杀伤与细胞辅助功能的组合也提供了一种新的和有效的策略，以减轻生物材料上的细菌感染和生物膜形成，并在骨科应用中具有很大的潜力。
Periprosthetic joint infection (PJI) is one of the formidable and recalcitrant complications after orthopedic surgery, and inhibiting biofilm formation on the implant surface is considered crucial to prophylaxis of PJI. However, it has recently been demonstrated that free-floating biofilm-like aggregates in the local body fluid and bacterial colonization on the implant and peri-implant tissues can coexist and are involved in the pathogenesis of PJI. An effective surface with both contact-killing and release-killing antimicrobial capabilities can potentially abate these concerns and minimize PJI caused by adherent/planktonic bacteria. Herein, Ag nanoparticles (NPs) are embedded in titania (TiO2) nanotubes by anodic oxidation and plasma immersion ion implantation (PIII) to form a contact-killing surface. Vancomycin is then incorporated into the nanotubes by vacuum extraction and lyophilization to produce the release-killing effect. A novel clinical PJI model system involving both in vitro and in vivo use of methicillin-resistantStaphylococcus aureus(MRSA) ST239 is established to systematically evaluate the antibacterial properties of the hybrid surface against planktonic and sessile bacteria. The vancomycin-loaded and Ag-implanted TiO2nanotubular surface exhibits excellent antimicrobial and antibiofilm effects against planktonic/adherent bacteria without appreciable silver ion release. The fibroblasts/bacteria cocultures reveal that the surface can help fibroblasts to combat bacteria. We first utilize the nanoarchitecture of implant surface as a bridge between the inorganic bactericide (Ag NPs) and organic antibacterial agent (vancomycin) to achieve total victory in the battle of PJI. The combination of contact-killing and release-killing together with cell-assisting function also provides a novel and effective strategy to mitigate bacterial infection and biofilm formation on biomaterials and has large potential in orthopedic applications.