SENSITIVE COLORIMETRIC METHOD FOR FORMALDEHYDE
SENSITIVE COLORIMETRIC METHOD FOR FORMALDEHYDE
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DOI：
10.1016/0003-2697(75)90205-5
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发表时间：
1975-01-01
影响因子：
2.9
通讯作者：
WILSON, JT
中科院分区：
文献类型：
--
作者：
CHRASTIL, J;WILSON, JT
在硫酸和铁、镍、钴存在下，甲醛与色氨酸反应生成紫色，最大吸收波长为575 nm。利用该反应可以用分光光度法测得甲醛的纳米分子，这也是一种非常特异的反应。其他氨基酸、糖和相关化合物、高醛和重金属不发生反应。3-取代吲哚给出相似的颜色，但强度较小。色氨酸反应可以用来测量利用肝脏和其他组织的部分生化反应形成的甲醛。
Formaldehyde reacts with tryptophan in the presence of sulfuric acid and iron, nickel, or cobalt and gives a violet color with absorption maximum at 575 nm. Nanomoles of formaldehyde can be estimated spectrophotometrically by using this reaction which is also very specific. Other aminoacids, sugars and related compounds, higher aldehydes, and heavy metals do not react. 3-Substituted indoles give similar colors of less intensity. The tryptophan reaction can be used to measure formaldehyde formed from biochemical reactions using fractions of liver and other tissues.