Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules
Ultrastructural localization of the high molecular weight proteins associated with in vitro-assembled brain microtubules
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与体外组装脑微管相关的高分子量蛋白的超微结构定位
DOI：
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发表时间：
1975
影响因子：
7.8
通讯作者：
J. Rosenbaum
中科院分区：
文献类型：
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作者：
W. Dentler;S. Granett;J. Rosenbaum
通过体外组装从脑提取物中分离的微管 (1, 19, 23) 主要由两种微管蛋白和两种高分子量蛋白（微管相关蛋白 [MAPS] 1 和 2）组成 (2,5,7,20)。最近，研究表明，体外组装的脑微管（神经管）涂有细丝（5, 7），其与原位附着在神经管上的细丝（4, 15, 21, 24, 25）相似，并且表明这些细丝由较高分子量的MAP组成（5, 7, 12）。在这项研究中，微管在存在和不存在 MAP 的情况下组装，并通过电子显微镜检查微管的薄切片。结果表明，丝仅出现在存在 MAP 的情况下组装的微管上，因此得出结论，丝是由高分子量 MAP 组成的。
Microtubules isolated from brain extracts by in vitro assembly (1, 19, 23) are composed principally of two tubulins and two high molecular weight proteins (microtubule-associated proteins [MAPS] 1 and 2) (2,5,7,20). Recently, it was demonstrated that in vitro-assembled brain microtubules (neurotubules) are coated with filaments (5, 7) which are similar to the filaments attached to neurotubules in situ (4, 15, 21, 24, 25), and it was suggested that the filaments are composed of the higher molecular weight MAPs (5, 7, 12). In this study, microtubules were assembled in the presence and absence of the MAPs, and thin sections of the microtubules were examined by electron microscopy. The results show that the filaments only occur on microtubules assembled in the presence of the MAPs and it is therefore concluded that the filaments are composed of the high molecular weight MAP's.