INBRED AND WILD MICE CARRY IDENTICAL DELETIONS IN THEIR E-ALPHA-MHC GENES
INBRED AND WILD MICE CARRY IDENTICAL DELETIONS IN THEIR E-ALPHA-MHC GENES
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DOI：
10.1002/j.1460-2075.1985.tb02326.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
MATHIS, DJ
中科院分区：
文献类型：
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作者：
DEMBIC, Z;AYANE, M;MATHIS, DJ
几种近交系和一定比例的野生小鼠在E α中存在缺失小鼠主要组织相容性复合体（H-2）基因。该突变阻止了E α的转录。基因并因此功能性表达E α E β。二聚体在细胞表面。选择两种菌株以更精确地定位该缺失。BALB.B是携带BALB/c背景的H-2b单倍型的同源系。CRO 435是来自埃及开罗附近捕获的野生小鼠的远交种;它携带H-2 w37单倍型，包括空值。图形 **。等位基因，以及H-2连锁的t复合体（tTuw 7）中的半致死突变。从这2个菌株中分离出含有跨越E α的片段的基因组克隆。缺失，并对断点区域进行测序。两个菌株中的缺失是相同的，跨越627 bp [碱基对]，其包括E α的启动子区和信号肽外显子。基因有限的序列比较表明.**图形 **。BALB的等位基因，B与.** 的关系更为密切。图形 **。比这两个都是E α-等位基因的CRO 435更高。表达子等位基因，.**图形 **。在不同的近交系和小鼠中的Eo缺失很可能是相同的起源，并且由于与t复合体的连锁，显然在整个小鼠群体中传播。
Several inbred strains and a certain percentage of wild mice bear a deletion in the E.alpha. gene of the mouse major histocompatibility complex (H-2). This mutation prevents transcription of the E.alpha. gene and hence functional expression of the E.alpha.E.beta. dimer on the cell surface. Two strains were selected for a more precise localization of this deletion. BALB.B is a congenic line carrying the H-2b haplotype of the BALB/c background. CRO435 is an outbred stock derived from a wild mouse captured near Cairo, Egypt; it carries the H-2w37 haplotype including a null .**GRAPHIC**. allele, as well as semi-lethal mutations in the H-2 linked t complex (tTuw7). From these 2 strains genomic clones were isolated that contain fragments spanning the E.alpha. deletion, and have sequenced the breakpoint region. The deletions in the 2 strains are identical, spanning 627 bp [base pair] which include the promotor region and the signal peptide exon of the E.alpha. gene. Limited sequence comparison suggests that the .**GRAPHIC**. allele of BALB.B is more closely related to the .**GRAPHIC**. allele of CRO435 than both of these are to an E.alpha.-expressor allele, .**GRAPHIC**. The Eo deletions in the various inbred strains and mice are probably of the same origin, and apparently were disseminated throughout the mouse population because of linkage to the t complex.