cis-Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cells.
cis-Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cells.
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DOI：
10.1091/mbc.e12-01-0034
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发表时间：
2012-08
影响因子：
3.3
通讯作者：
Nakano A
中科院分区：
文献类型：
--
作者：
Ito Y;Uemura T;Shoda K;Fujimoto M;Ueda T;Nakano A
特别是顺式高尔基体蛋白积累在新的点状结构接近ERES的BFA处理在烟草BY-2细胞。去除BFA后，这些结构首先重新组装形成顺式高尔基体，高尔基体堆叠以顺式到反式顺序再生。这表明点状结构作为高尔基体再生的支架。在许多真核细胞中，高尔基体形成一堆池。然而，人们对这种堆叠结构是如何形成和维持的知之甚少。为了解决这个问题，植物细胞提供了一个适合于活体成像方法的系统，因为单个高尔基体堆叠在细胞质中被很好地分离。我们建立了烟草BY-2细胞系，表达多种高尔基体标记不同的荧光蛋白标记，并观察其响应布雷菲德菌素A（BFA）处理和BFA去除。BFA治疗破坏顺式，内侧和反式脑池，但引起不同的重新定位模式取决于蛋白质检查。内侧和反式高尔基体蛋白，以及一个顺式高尔基体蛋白，被吸收到内质网（ER），但其他两个顺式高尔基体蛋白形成小的点状结构。去除BFA后，这些斑点首先合并，然后高尔基体堆叠从它们以顺式到反式顺序再生。我们认为，这些结构具有类似于ER-高尔基体中间隔室的性质，并作为高尔基体再生的支架发挥作用。
Particular cis-Golgi proteins accumulate in novel punctate structures close to ERES by BFA treatment in tobacco BY-2 cells. These structures reassemble first to form cis-Golgi after BFA removal, and the Golgi stacks regenerate in the cis-to-trans order. This indicates that the punctate structures act as the scaffold for Golgi regeneration. The Golgi apparatus forms stacks of cisternae in many eukaryotic cells. However, little is known about how such a stacked structure is formed and maintained. To address this question, plant cells provide a system suitable for live-imaging approaches because individual Golgi stacks are well separated in the cytoplasm. We established tobacco BY-2 cell lines expressing multiple Golgi markers tagged by different fluorescent proteins and observed their responses to brefeldin A (BFA) treatment and BFA removal. BFA treatment disrupted cis, medial, and trans cisternae but caused distinct relocalization patterns depending on the proteins examined. Medial- and trans-Golgi proteins, as well as one cis-Golgi protein, were absorbed into the endoplasmic reticulum (ER), but two other cis-Golgi proteins formed small punctate structures. After BFA removal, these puncta coalesced first, and then the Golgi stacks regenerated from them in the cis-to-trans order. We suggest that these structures have a property similar to the ER-Golgi intermediate compartment and function as the scaffold of Golgi regeneration.