GENE-EXPRESSION IN SUSCEPTIBLE AND DISEASE RESISTANT INTERACTIONS OF PEAS INDUCED WITH FUSARIUM-SOLANI PATHOGENS AND CHITOSAN
GENE-EXPRESSION IN SUSCEPTIBLE AND DISEASE RESISTANT INTERACTIONS OF PEAS INDUCED WITH FUSARIUM-SOLANI PATHOGENS AND CHITOSAN
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DOI：
10.1016/0048-4059(85)90053-0
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
HADWIGER, LA
中科院分区：
文献类型：
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作者：
FRISTENSKY, B;RIGGLEMAN, RC;HADWIGER, LA
建立植物抗病性的分子基础的第一步可能是鉴定其表达密切跟随感染过程中发生的事件的基因。从之前的 cDNA 文库筛选中获得了 9 个豌豆克隆，这些克隆是由接种镰刀菌 8 小时后诱导的豌豆 mRNA 单一群体开发的。 sp。菜豆。在用相容或不相容形式的茄病镰刀菌或壳聚糖处理豌豆后的 48 小时感染过程中，比较了与这些克隆同源的 RNA 的转录和积累，壳聚糖是真菌细胞壁的次要成分，已知可诱导抗病性。根据基因表达，豌豆组织的不相容反应与相容反应有明显区别。被命名为第一组克隆的三个克隆基因在抗病组织中显示出它们的RNA的大量诱导，这与细胞学观察到的抗性在时间上相关。与不相容反应相比，在相容反应中，I 组基因的激活通常较弱或延迟，并且在接种后 12-24 小时被抑制。与第二组克隆同源的基因仅部分符合抗性基因的预期活性。我们提出，选择诱导时间过程与植物组织对感染的反应相匹配的克隆基因是一种有用的初步筛选技术，以获得用于跨物种转化实验的候选基因。
A first step for establishing the molecular basis of disease resistance in plants may be to identify genes whose expression closely follows the events occurring during infection. Nine pea clones were available from a prior screening of a cDNA library, developed from a single population of pea mRNA induced 8 h following inoculation with Fusarium solani f. sp. phaseoli. Transcription and accumulation of RNA homologous to these clones were compared over a 48 h infection course following the treatment of pea pods with either compatible or incompatible forms of F. solani or chitosan, a minor component of the fungal cell wall which is known to induce disease resistance. The incompatible reaction of the pea tissue is clearly distinguished from the compatible reaction on the basis of gene expression. Three of the cloned genes, designated Group I clones, show a large induction of their RNAs in disease resisting tissue which temporally correlates with the resistance observed cytologically. In the compatible reaction activation of Group I genes is commonly weaker or delayed, and is suppressed 12-24 h after inoculation, compared with the incompatible reaction. Genes homologous with Group II clones showed only a partial fit to the activity expected for resistance genes. We propose that selecting cloned genes whose time course of induction matches the response of plant tissue to infection is a useful preliminary screening technique towards obtaining gene candidates for cross-species transformation experiments.