Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by Cupriavidus sp DT-1
Biodegradation of chlorpyrifos and 3,5,6-trichloro-2-pyridinol by Cupriavidus sp DT-1
复制标题
Cupriavidus sp. 生物降解毒死蜱和 3,5,6-三氯-2-吡啶醇。
DOI：
10.1016/j.biortech.2012.09.116
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发表时间：
2013-01-01
影响因子：
11.4
通讯作者：
Li, Shunpeng
中科院分区：
文献类型：
--
作者：
Lu, Peng;Li, Qinfen;Li, Shunpeng
一种细菌菌株，Cupriavidus sp。分离并表征了能够降解毒死蜱和 3,5,6-三氯-2-吡啶醇 (TCP) 并使用这些化合物作为唯一碳源的 DT-1。降解途径研究表明，毒死蜱首先水解为TCP，随后脱氯为2-吡啶醇，然后吡啶环裂解并进一步降解。 mpd 基因编码负责毒死蜱水解为 TCP 的酶，已在大肠杆菌 BL21 中克隆并表达。用菌株DT-1接种受毒死蜱污染的土壤，毒死蜱和TCP的降解率分别为100%和94.3%，而未接种土壤的降解率分别为28.2%和19.9%。这一发现表明菌株 DT-1 有潜力用于毒死蜱污染环境的生物修复。 (C) 2012 Elsevier Ltd. 保留所有权利。
A bacterial strain, Cupriavidus sp. DT-1, capable of degrading chlorpyrifos and 3,5,6-trichloro-2-pyridinol (TCP) and using these compounds as sole carbon source was isolated and characterized. Investigation of the degradation pathway showed that chlorpyrifos was first hydrolyzed to TCP, successively dechlorinated to 2-pyridinol, and then subjected to the cleavage of the pyridine ring and further degradation. The mpd gene, encoding the enzyme responsible for chlorpyrifos hydrolysis to TCP, was cloned and expressed in Escherichia coli BL21. Inoculation of chlorpyrifos-contaminated soil with strain DT-1 resulted in a degradation rate of chlorpyrifos and TCP of 100% and 94.3%, respectively as compared to a rate of 28.2% and 19.9% in uninoculated soil. This finding suggests that strain DT-1 has potential for use in bioremediation of chlorpyrifos-contaminated environments. (C) 2012 Elsevier Ltd. All rights reserved.