Role for sperm in spatial patterning of the early mouse embryo
Role for sperm in spatial patterning of the early mouse embryo
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DOI：
10.1038/35054069
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发表时间：
2001-01-25
期刊：
影响因子：
64.8
通讯作者：
Zernicka-Goetz, M
中科院分区：
文献类型：
--
作者：
Piotrowska, K;Zernicka-Goetz, M
尽管明显缺乏决定细胞命运的决定因素，但小鼠胚胎的空间模式在发育早期是明显的。着床后卵柱的轴可以追溯到着床前胚泡的组织（1）。这反过来又反映了卵裂期胚胎的组织结构和合子的动物-植物轴（2，3）。这些发现表明，正常发育的卵裂模式可能参与指定未来的胚胎轴;然而，这种模式如何以及何时建立尚不清楚。在许多动物卵子中，精子进入位置为胚胎模式提供了线索（4-6），但直到现在还没有在哺乳动物中发现这种作用。在这里，我们表明，精子的入口位置预测的小鼠卵子的初始分裂的平面，并可以定义未来的胚泡的胚胎和胚外的一半。此外，继承精子进入位置的细胞获得了分裂优势，并且倾向于先于其姐妹细胞分裂。由于细胞身份反映了早期卵裂的时间，这些事件共同塑造了胚泡，胚泡的组织在植入后将转化为轴向图案。我们提出了一个轴向发育模型，该模型将这些发现与小鼠胚胎的调节性质相适应。
Despite an apparent lack of determinants that specify cell fate, spatial patterning of the mouse embryo is evident early in development. The axis of the post-implantation egg cylinder can be traced back to organization of the pre-implantation blastocyst(1). This in turn reflects the organization of the cleavage-stage embryo and the animal-vegetal axis of the zygote(2,3). These findings suggest that the cleavage pattern of normal development may be involved in specifying the future embryonic axis; however, how and when this pattern becomes established is unclear. In many animal eggs, the sperm entry position provides a cue for embryonic patterning(4-6), but until now no such role has been found in mammals. Here we show that the sperm entry position predicts the plane of initial cleavage of the mouse egg and can define embryonic and abembryonic halves of the future blastocyst. In addition, the cell inheriting the sperm entry position acquires a division advantage and tends to cleave ahead of its sister. As cell identity reflects the timing of the early cleavages, these events together shape the blastocyst whose organization will become translated into axial patterning after implantation. We present a model for axial development that accommodates these findings with the regulative nature of mouse embryos.