Use of a novel microfluidic disk in the analysis of single-cell viability and the application to Jurkat cells
Use of a novel microfluidic disk in the analysis of single-cell viability and the application to Jurkat cells
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DOI：
10.1016/j.jbiosc.2011.03.016
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发表时间：
2011-07-01
影响因子：
2.8
通讯作者：
Matoba, Kenji
中科院分区：
文献类型：
--
作者：
Kubo, Izumi;Furutani, Shunsuke;Matoba, Kenji
将Jurkat细胞捕获在新型盘形细胞分离装置的微室中并用Cellstain染色。大约90%的细胞是活的。在以4500 rpm旋转30 s后，用分支微通道分离单细胞，表明活的单细胞可以被捕获在微室中。(C)2011年，生物技术学会，日本。All rights reserved.
Jurkat cells were trapped in the microchambers of a novel disk-shaped cell separation device and stained with Cellstain. Approximately 90% of the cells were living. Single cells were isolated with a branching microchannel after rotation at 4500 rpm for 30 s, demonstrating that a living single cell could be trapped in the microchambers. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.