Collagen fibril organization within rat vertebral bone modified with metastatic involvement
Collagen fibril organization within rat vertebral bone modified with metastatic involvement
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DOI：
10.1016/j.jsb.2017.06.008
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发表时间：
2017-08-01
影响因子：
3
通讯作者：
Whyne, Carl
中科院分区：
文献类型：
--
作者：
Burke, Mikhail;Golaraei, Ahmad;Whyne, Carl
转移性累及降低了椎骨的机械完整性，然而其对骨组织的主要成分胶原-I原纤维基质的结构特征的具体影响尚未得到充分表征。将雌性无胸腺大鼠分别接种HeLa或Ace-1癌细胞系，产生溶骨性或混合（溶骨性和成骨细胞）转移。接种和处死大鼠以取出椎骨之间最多允许21天。利用线性极化输入、极化输出（PIPO）二次谐波发生（SHG）和透射电子显微镜（TEM）成像来评估转移参与对胶原纤维组织的影响。与健康对照组相比，转移灶附近骨中典型胶合板图案或平行堆积结构的偏离观察结果增加，平均测量敏感性比（与分析组织区域中平面内与平面外原纤维的相对程度相关）增加，表明原纤维组织发生变化。特别是，在肿瘤诱导的成骨细胞骨生长的胶原蛋白-I原纤维没有粘附到胶合板图案或平行包装结构中看到健康的板层骨，表现出更高的易感性比和程度的原纤维紊乱。在先前的研究中，测量的易感性比率与转移性骨组织的硬度和模量之间建立了负相关性。表征组织水平性质的变化是确定存在转移性疾病时骨质量及其对材料行为的潜在影响的关键。(C)2017爱思唯尔公司All rights reserved.
Metastatic involvement diminishes the mechanical integrity of vertebral bone, however its specific impact on the structural characteristics of a primary constituent of bone tissue, the collagen-I fibril matrix, has not been adequately characterized. Female athymic rats were inoculated with HeLa or Ace-1 cancer cells lines producing osteolytic or mixed (osteolytic & osteoblastic) metastases respectively. A maximum of 21 days was allowed between inoculation and rat sacrifice for vertebrae extraction. Linear polarization-in, polarization-out (PIPO) second harmonic generation (SHG) and transmission electron microscopy (TEM) imaging was utilized to assess the impact of metastatic involvement on collagen fibril organization. Increased observations of deviations in the typical plywood motif or a parallel packing structure and an increased average measured susceptibility ratio (related to relative degree of in-plane vs. out-plane fibrils in the analyzed tissue area) in bone adjacent to metastatic involvement was indicative of change in fibrilar organization compared to healthy controls. In particular, collagen-I fibrils in tumour induced osteoblastic bone growth showed no adherence to the plywood motif or parallel packing structure seen in healthy lamellar bone, exhibiting a much higher susceptibility ratio and degree of fibril disorder. Negative correlations were established between measured susceptibility ratios and the hardness and modulus of metastatic bone tissue assessed in a previous study. Characterizing modifications in tissue level properties is key in defining bone quality in the presence of metastatic disease and their potential impact on material behaviour. (C) 2017 Elsevier Inc. All rights reserved.