Colonization of Streptococcus mutans and Streptococcus sobrinus genotypes and caries development in children to mothers harboring both species
Colonization of Streptococcus mutans and Streptococcus sobrinus genotypes and caries development in children to mothers harboring both species
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DOI：
10.1159/000075932
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发表时间：
2004-01-01
期刊：
影响因子：
4.2
通讯作者：
Emilson, CG
中科院分区：
文献类型：
--
作者：
Lindquist, B;Emilson, CG
人类早期感染变形链球菌的主要途径是母婴垂直传播。这项纵向研究的目的是了解母亲同时携带变形链球菌和远缘链球菌的儿童中变形链球菌和远缘链球菌的获得性、分布和持久性，并研究龋患率与这些细菌定植的关系。在孩子出生的头7年里，对15对母子进行了跟踪调查。刺激性唾液样本取自母亲和孩子。还从儿童的牙齿和舌头上采集了菌斑样本。通过培养技术结合基因组指纹图谱和杂交技术对样品进行分析。使用社区诊所的龋病登记记录，在抽样次数上对龋病体验进行评估。在这7年期间，15名儿童中有10名感染了变形链球菌。其中只有4个同时被变形链球菌和远缘链霉菌定居，尽管它们的母亲同时庇护了这两个物种。在2名儿童中，远缘链球菌的发现晚于变形链球菌。在这些儿童中总共发现了26种基因型，其中9种与他们的母亲完全相同。发现了新的基因类型和得失模式，特别是在儿童中，在他们的母亲中也是如此。首先对这两个物种呈阳性反应的牙组是乳磨牙。在研究期间，患龋齿的几率很低，有8名儿童没有龋齿。
The major route of early acquisition of mutans streptococci in humans is a vertical transmission from mother to child. The purpose of this longitudinal study was to examine the acquisition, distribution and persistence of Streptococcus mutans and Streptococcus sobrinus in children whose mothers harbored both species and to study the caries incidence in relation to colonization of these bacteria. Fifteen mother-child pairs were followed during the child's first 7 years. Stimulated salivary samples were taken from the mothers and the children. Plaque samples were also collected from the teeth and the tongue of the children. The samples were analyzed by cultivating techniques together with genomic fingerprinting and hybridizing. The caries experience was evaluated on the sampling occasions and retrospectively using the records of caries registrations from the community clinics. During the 7-year period 10 of the 15 children acquired mutans streptococci. Only 4 of them were colonized by both S. mutans and S. sobrinus despite the fact that their mothers harbored both species. In 2 of the children S. sobrinus was found later than S. mutans. A total of 26 genotypes were found in the children and 9 of them were identical to their mothers. New genotypes and a gain-loss pattern were noted especially in the children but also in their mothers. The groups of teeth first positive for the two species were the deciduous molars. The caries experience was low during the study period with 8 children showing no caries.