THE FOCUSING POSITIONS OF POLYPEPTIDES IN IMMOBILIZED PH GRADIENTS CAN BE PREDICTED FROM THEIR AMINO-ACID-SEQUENCES
THE FOCUSING POSITIONS OF POLYPEPTIDES IN IMMOBILIZED PH GRADIENTS CAN BE PREDICTED FROM THEIR AMINO-ACID-SEQUENCES
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DOI：
10.1002/elps.11501401163
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发表时间：
1993-10-01
期刊：
影响因子：
2.9
通讯作者：
HOCHSTRASSER, D
中科院分区：
文献类型：
--
作者：
BJELLQVIST, B;HUGHES, GJ;HOCHSTRASSER, D
在变性条件下，确定了固定的29个多肽的狭窄范围内的pH梯度的聚焦位置。从其氨基酸序列计算得出的蛋白质的等电点具有良好的精度，实验确定的PI值。我们显示了能够预测二维凝胶中已知结构蛋白的位置的优势。
The focusing Positions in narrow range immobilized pH gradients of 29 polypeptides of known amino acid sequence were determined under denaturing conditions. The isoelectric points of the proteins calculated from their amino acid sequences matched with good accuracy the experimentally determined pI values. We show the advantages of being able to predict the position of a protein of known structure within a two-dimensional gel.