Protective effect of Korean Red Ginseng against chemotherapeutic drug-induced premature catagen development assessed with human hair follicle organ culture model.
Protective effect of Korean Red Ginseng against chemotherapeutic drug-induced premature catagen development assessed with human hair follicle organ culture model.
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DOI：
10.1016/j.jgr.2015.07.004
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发表时间：
2016-04
影响因子：
6.3
通讯作者：
Lee WS
中科院分区：
文献类型：
--
作者：
Keum DI;Pi LQ;Hwang ST;Lee WS
化疗引起的脱发（CIA）是接受化疗的患者最令人痛苦的副作用之一。本研究评价了韩国红参（KRG）对CIA的保护作用，在一个完善的体外人毛囊器官培养模型，因为它发生在体内。我们在人毛囊器官培养模型中研究了KRG在用关键的环磷酰胺代谢物4-氢过氧环磷酰胺（4-HC）治疗期间是否可以预防过早的毛囊营养不良。4-HC抑制人毛发生长，诱导毛发过早退化期发育，抑制毛发基质角质形成细胞增殖并刺激其凋亡。此外，4-HC增加p53和Bax蛋白表达，降低Bcl 2蛋白表达。预处理与KRG保护4-HC诱导的毛发生长抑制和过早的退化期发展。KRG还抑制4-HC诱导的抑制基质角质形成细胞增殖和刺激基质角质形成细胞凋亡。此外，KRG恢复4-HC诱导的p53和Bax/Bcl 2表达。总体而言，我们的研究结果表明，KRG可以通过调节p53和Bax/Bcl 2的表达来防止4-HC诱导的过早退化期的发展。
Chemotherapy-induced alopecia (CIA) is one of the most distressing side effects for patients undergoing chemotherapy. This study evaluated the protective effect of Korean Red Ginseng (KRG) on CIA in a well-established in vitro human hair follicle organ culture model as it occurs in vivo. We examined whether KRG can prevent premature hair follicle dystrophy in a human hair follicle organ culture model during treatment with a key cyclophosphamide metabolite, 4-hydroperoxycyclophosphamide (4-HC). 4-HC inhibited human hair growth, induced premature catagen development, and inhibited proliferation and stimulated apoptosis of hair matrix keratinocytes. In addition, 4-HC increased p53 and Bax protein expression and decreased Bcl2 protein expression. Pretreatment with KRG protected against 4-HC-induced hair growth inhibition and premature catagen development. KRG also suppressed 4-HC-induced inhibition of matrix keratinocyte proliferation and stimulation of matrix keratinocyte apoptosis. Moreover, KRG restored 4-HC-induced p53 and Bax/Bcl2 expression. Overall, our results indicate that KRG may protect against 4-HC-induced premature catagen development through modulation of p53 and Bax/Bcl2 expression.