Reciprocal changes of H3K27ac and H3K27me3 at the promoter regions of the critical genes for endometrial decidualization
Reciprocal changes of H3K27ac and H3K27me3 at the promoter regions of the critical genes for endometrial decidualization
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DOI：
10.2217/epi-2018-0006
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发表时间：
2018-09-01
期刊：
影响因子：
3.8
通讯作者：
Hata, Kenichiro
中科院分区：
文献类型：
--
作者：
Katoh, Noriko;Kuroda, Keiji;Hata, Kenichiro
目的：蜕膜化对于胚胎着床和胎盘发育至关重要。我们的目的是获得原代子宫内膜基质细胞（ESC）和体外蜕膜细胞的转录组和表观基因组图谱。材料和方法：从人子宫内膜组织中分离出的 ESC 未经处理 (D0)，或在 8-溴-cAMP 和黄体酮存在下蜕膜 4 天 (D4) 和 8 天 (D8)。结果：在所检查的表观遗传修饰（DNA 甲基化、H3K27ac、H3K9me3 和 H3K27me3）中，蜕膜化后 H3K27ac 模式变化最为显着，与基因表达变化具有中等相关性。蜕膜化时上调和下调的基因子集与其启动子区域的 H3K27ac 和 H3K27me3 修饰的相互变化相关，并且富含蜕膜化必需的基因，例如 WNT4、ZBTB16、PROK1 和 GREB1。结论：我们的数据集有助于进一步阐明蜕膜化的分子机制。
Aim: Decidualization is essential for embryo implantation and placental development. We aimed to obtain transcriptome and epigenome profiles for primary endometrial stromal cells (ESCs) and in vitro decidualized cells. Materials & methods: ESCs isolated from human endometrial tissues remained untreated (D0), or decidualized for 4 days (D4) and 8 days (D8) in the presence of 8-bromo-cAMP and progesterone. Results: Among the epigenetic modifications examined (DNA methylation, H3K27ac, H3K9me3 and H3K27me3), the H3K27ac patterns changed most dramatically, with a moderate correlation with gene expression changes, upon decidualization. Subsets of up- and down-regulated genes upon decidualization were associated with reciprocal changes of H3K27ac and H3K27me3 modifications at their promoter region, and were enriched with genes essential for decidualization such as WNT4, ZBTB16, PROK1 and GREB1. Conclusion: Our dataset is useful to further elucidate the molecular mechanisms underlying decidualization.