Preparation and evaluation of a novel monolithic column containing double octadecyl chains for reverse-phase micro high performance liquid chromatography
Preparation and evaluation of a novel monolithic column containing double octadecyl chains for reverse-phase micro high performance liquid chromatography
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新型反相微量高效液相色谱双十八烷基链整体柱的制备与评价
DOI：
10.1016/j.chroma.2014.04.036
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发表时间：
2014-06-06
影响因子：
4.1
通讯作者：
Jiang, Zhengjin
中科院分区：
文献类型：
--
作者：
Duan, Qianqian;Liu, Chusheng;Jiang, Zhengjin
以2-甲基-1-丙醇和1，4-丁二醇为致孔剂，通过3-甲基丙烯酰基-3-氧丙-3-（N，N-双十八烷基氨基甲酰基）-丙酸酯（AOD）和乙二醇二甲基丙烯酸酯（EDMA）的热引发共聚合反应，制备了一种新型的含有双长烷基链的反相整体固定相。对聚合过程进行了优化，并对最终的整体柱进行了良好的渗透性、稳定性和柱效观察。该色谱柱也显示出良好的长期稳定性和重现性。亚甲基选择性表现出典型的反相特征。优化后的聚（AOD-co-EDMA）整体柱对多环芳烃、生育酚和烷基苯酮等非极性待测物表现出良好的选择性。还观察到完整蛋白质的良好分离。(C)2014爱思唯尔有限公司版权所有。
A novel reverse-phase monolithic stationary phase containing double long alkyl chains was prepared based on the thermally initiated co-polymerization of 3-methylacryloyl-3-oxapropyl-3-(N,N-dioctadecylcarbamoyl)-propionate (AOD) and ethylene glycol dimethacrylate (EDMA) in the presence of 2-methyl-1-propanol and 1,4-butanediol as the selected porogens. The polymerization was carefully optimized and good permeability, stability and column efficiency were observed for the final monolithic columns. The column also showed good long term stability and reproducibility. The methylene selectivity demonstrates typical reversed phase characteristics. The optimized poly (AOD-co-EDMA) monolith exhibited good selectivity for a range of non-polar test analytes such as PAHs, tocopherols and alkylphenones. A good separation of intact proteins was also observed. (C) 2014 Elsevier B.V. All rights reserved.