Chemotaxis by mouse macrophage cell lines.
Chemotaxis by mouse macrophage cell lines.
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小鼠巨噬细胞系的趋化作用。
DOI：
10.4049/jimmunol.126.6.2194
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发表时间：
1981
影响因子：
4.4
通讯作者：
E. Leonard
中科院分区：
文献类型：
--
作者：
R. Aksamit;W. Falk;E. Leonard
测试了五种小鼠巨噬细胞系的趋化性和吞噬作用。所有5种细胞系均摄入兔IgG抗Forssman抗体包被的绵羊红细胞，并对内毒素激活的小鼠血清（EAMS）和淋巴细胞衍生的趋化因子表现出趋化性。两个细胞系进行了测试的f-Met-Leu-Phe的趋化性，都没有响应。4个细胞系（RAW 264、RAW 309 CR、PU 5 - 1 R和WR19M.1）表现出对C5 a的趋化性。这些细胞系在向EAMS迁移之前显示1至2小时的滞后，并且趋化性依赖于细胞密度。当每mm 2滤器表面存在少于10（3）个细胞时，少于10%的细胞迁移;然而，在5 × 10（3）个细胞/mm 2的密度下，50 - 70%的细胞迁移。WEHI-3与其他细胞系的不同之处在于，对C5 a没有趋化性，迁移到EAMS没有可检测的滞后，并且趋化性没有细胞密度依赖性。将这些趋化特性与文献中报道的小鼠巨噬细胞和单核细胞的趋化特性进行比较表明，RAW 264、RAW 309 CR、PU 5 - 1 R和WR19M.1具有与小鼠驻留巨噬细胞相似的特性，而WEHI-3可能具有小鼠单核细胞的一些特性。
Five mouse macrophage cell lines were tested for chemotaxis and phagocytosis. All 5 cell lines ingested sheep red cells coated with rabbit IgG anti-Forssman antibody and exhibited chemotaxis to endotoxin-activated mouse serum (EAMS) and lymphocyte-derived chemotactic factor. Two cell lines were tested for chemotaxis to f-Met-Leu-Phe and neither responded. Four of the cell lines (RAW264, RAW309CR, PU5-1R, and WR19M.1) exhibited chemotaxis to C5a. These cell lines displayed a 1- to 2-hr lag before migrating toward EAMS, and chemotaxis was dependent upon cell density. When fewer than 10(3) cells were present per mm2 of filter surface, less than 10% of the cells migrated; however, at a density of 5 X 10(3) cells/mm2 50 to 70% of the cells migrated. WEHI-3 differed from the other cell lines in that there was no chemotaxis to C5a, migration to EAMS did not have a detectable lag, and there was no cell density dependence for chemotaxis. Comparison of these chemotactic properties with those reported in the literature for mouse macrophages and monocytes suggests that RAW264, RAW309CR, PU5-1R, and WR19M.1 have properties that are similar to those of mouse resident macrophages, whereas WEHI-3 may have some of the properties of mouse monocytes.