Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus
Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus
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冷冻电镜显示 dsRNA 病毒内的聚合酶结构和非缠绕基因组
DOI：
10.1126/science.aaa4938
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发表时间：
2015-09-18
期刊：
影响因子：
56.9
通讯作者：
Cheng, Lingpeng
中科院分区：
文献类型：
--
作者：
Liu, Hongrong;Cheng, Lingpeng
双链RNA（dsRNA）病毒具有分段的dsRNA基因组和许多包封在衣壳中的RNA依赖性RNA聚合酶（RdRps）。到目前为止，衣壳内基因组和RdRps的精确结构还不清楚。在这里，我们报告的RdRps和相关的RNA的结构内非转录和转录cypoviruses（NCPV和TCPV，分别），使用冷冻电子显微镜（cryo-EM）和一个冷冻不匹配的重建方法的组合。在NCPV和TCPV中，RdRps和相关RNA似乎表现出伪D-3对称组织。然而，发现RdRps和基因组RNA之间的分子相互作用在这些状态下是不同的。我们的工作提供了深入了解的复制和转录的dsRNA病毒的机制，并铺平了一条道路，为结构确定较低的对称性复杂的封闭在较高的对称性结构。
Double-stranded RNA (dsRNA) viruses possess a segmented dsRNA genome and a number of RNA-dependent RNA polymerases (RdRps) enclosed in a capsid. Until now, the precise structures of genomes and RdRps within the capsids have been unknown. Here we report the structures of RdRps and associated RNAs within nontranscribing and transcribing cypoviruses (NCPV and TCPV, respectively), using a combination of cryo-electron microscopy (cryo-EM) and a symmetry-mismatch reconstruction method. The RdRps and associated RNAs appear to exhibit a pseudo-D-3 symmetric organization in both NCPV and TCPV. However, the molecular interactions between RdRps and the genomic RNA were found to differ in these states. Our work provides insight into the mechanisms of the replication and transcription in dsRNA viruses and paves a way for structural determination of lower-symmetry complexes enclosed in higher-symmetry structures.