PHOSPHOLIPID, ESTER-LINKED FATTY-ACID PROFILES AS REPRODUCIBLE ASSAYS FOR CHANGES IN PROKARYOTIC COMMUNITY STRUCTURE OF ESTUARINE SEDIMENTS
PHOSPHOLIPID, ESTER-LINKED FATTY-ACID PROFILES AS REPRODUCIBLE ASSAYS FOR CHANGES IN PROKARYOTIC COMMUNITY STRUCTURE OF ESTUARINE SEDIMENTS
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DOI：
10.1111/j.1574-6968.1985.tb01143.x
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发表时间：
1985-01-01
影响因子：
4.2
通讯作者：
WHITE, DC
中科院分区：
文献类型：
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作者：
GUCKERT, JB;ANTWORTH, CP;WHITE, DC
磷脂，酯连接的脂肪酸的配置文件显示底栖原核生物群落结构的变化，反映了文化的操作，都是定量和统计上显着的。脂肪酸结构，包括双键的位置和顺式/反式几何结构，在适当的衍生化后通过气相色谱/质谱法进行化学验证。当操作相同时，独立烧瓶的脂肪酸谱显示出可重现的变化，当用不同处理操作时，显示出显著差异。多不饱和脂肪酸的缺乏表明聚生体主要是原核的。不同处理的原核聚生体可以通过环丙基脂肪酸的比例和单不饱和脂肪酸的比例和几何形状来区分。
Phospholipid, ester-linked fatty acid profiles showed changes in benthic prokaryotic community structure reflecting culture manipulations that were both quantitative and statistically significant. Fatty acid structures, including the position and cis/trans geometry of double bonds, were chemically verified by gas chromatography/mass spectrometry after appropriate derivatization. The fatty acid profiles of independent flasks showed reproducible shifts when manipulated identically and significant differences when manipulated with different treatments. The a bsence of polyunsaturated fatty acids indicated that the consortia were predominantly prokaryotic. The prokaryotic consortia of different treatments could be differentiated by the proportions of cyclopropyl fatty acids and the proportions and geometry of monounsaturated fatty acids.