Orexigenic action of oral zinc: metabolomic analysis in the rat hypothalamus
Orexigenic action of oral zinc: metabolomic analysis in the rat hypothalamus
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DOI：
10.1080/09168451.2018.1516543
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发表时间：
2018-12-02
影响因子：
1.6
通讯作者：
Komai, Michio
中科院分区：
文献类型：
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作者：
Nishiuchi, Mika;Sakai, Kumiko;Komai, Michio
我们之前报道了雄性SD大鼠在早期喂锌缺乏饮食时口服锌的增氧作用，没有降低组织中的锌浓度。总的结论是口服锌而不是腹腔注射锌刺激短期缺锌大鼠的食物摄入。本研究采用GC-MS/ ms靶向代谢组学方法研究锌在大鼠下丘脑的促氧作用机制。选用4周龄雄性SD/Slc大鼠，在饲喂缺锌饲料2天后，每只大鼠口服或腹腔注射5ml生理盐水溶液中3mg硫酸锌。给药3小时后，处死大鼠，切除下丘脑进行分析。我们发现口服给药组3-氨基丙酸（β -丙氨酸）、次牛磺酸、多巴胺和生物素的浓度增加。根据这些结果的代谢组学分析，我们指出了进一步研究的方向。
We previously reported an orexigenic action of oral zinc administration in male Sprague-Dawley (SD) rats during an early stage of feeding with a zinc-deficient diet, without decreased zinc concentrations in tissues. The overall conclusion was that orally but not intraperitoneally administered zinc stimulates food intake in short-term zinc-deficient-diet fed rats. We here investigate the mechanism of the orexigenic action of zinc using GC-MS/MS-targeted metabolomic analysis in the rat hypothalamus. Four-week-old, male SD/Slc rats were used, and after 2 days of feeding with a zinc-deficient diet, 3 mg of ZnSO4 in 5 mL saline solution were administered to each rat either orally or intraperitoneally. Three hours after administration, the rats were sacrificed and the hypothalamus were excised and analyzed. We found that the oral administration group showed increased concentrations of 3-aminopropanoic acid (beta-alanine), hypotaurine, dopamine, and biotin. In light of metabolomic analysis of these results, we indicate directions for further research.