CHITIN SYNTHESIS AND SHEATH MORPHOGENESIS IN BRUGIA-MALAYI MICROFILARIAE
CHITIN SYNTHESIS AND SHEATH MORPHOGENESIS IN BRUGIA-MALAYI MICROFILARIAE
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DOI：
10.1016/0166-6851(85)90130-6
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发表时间：
1985-01-01
影响因子：
1.5
通讯作者：
PIESSENS, WF
中科院分区：
文献类型：
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作者：
FUHRMAN, JA;PIESSENS, WF
对马来丝蚴和怀孕的成年雌性进行了检查，以确定几丁质（聚-β（1.fwdarw.4）-连接的N-乙酰氨基葡萄糖）是否是微丝蚴鞘的结构成分。两种对β(1.fwdarw.4)连接的N-乙酰葡糖胺寡聚物具有特异性的凝集素与活微丝蚴的鞘结合。除虫脲是昆虫和甲壳类动物几丁质合成的有效抑制剂，它会导致怀孕的雌性蠕虫脱落鞘被截断的后代微丝蚴。可以从怀孕的雌性（但不是雄性）蠕虫中分离出类几丁质部分（不溶于热碱且对几丁质酶敏感）。该部分可以用放射性葡萄糖胺进行代谢标记，但这种标记会被除虫脲抑制。这些数据表明，几丁质合成对于这种寄生线虫的微丝蚴鞘形态发生至关重要。
Brugia malayi microfilariae and gravid adult females were examined to determine whether chitin (poly-.beta.(1 .fwdarw. 4)-linked N-acetylglucosamine) is a structural component of the microfilarial sheath. Two lectins which are specific for .beta.(1 .fwdarw. 4)-linked oligomers of N-acetylglucosamine bind to the sheaths of living microfilariae. Diflubenzuron, a potent inhibitor of chitin synthesis in insects and crustaceans, causes gravid female worms to shed progeny microfilariae with truncated sheaths. A chitin-like fraction (hot alkali-insoluble and chitinase-sensitive) can be isolated from gravid female (but not male) worms. This fraction can be metabolically labelled with radioactive glucosamine, but such labelling is inhibited by diflubenzuron. These data suggest that chitin synthesis is critical to microfilarial sheath morphogenesis in this parasitic nematode.