Flow-injection chemiluminescent immunoassay for α-fetoprotein based on epoxysilane modified glass microbeads
Flow-injection chemiluminescent immunoassay for α-fetoprotein based on epoxysilane modified glass microbeads
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DOI：
10.1016/j.jim.2006.02.006
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发表时间：
2006-05-30
影响因子：
2.2
通讯作者：
Ju, Huangxian
中科院分区：
文献类型：
--
作者：
Fu, Zhifeng;Hao, Chen;Ju, Huangxian
提出了一种基于透明免疫亲和反应器的甲胎蛋白定量流动注射化学发光免疫分析系统。用甲胎蛋白固定化环氧硅烷修饰玻璃微珠制备的反应器作为化学发光检测的免疫传感器。随着非竞争性免疫分析的正式实施，所提出的免疫传感器系统是一种低成本、灵活和快速的甲胎蛋白检测方法。将分析物甲胎蛋白与辣根过氧化物酶标记的甲胎蛋白抗体作为酶示踪剂离线孵卵后，将混合物注入反应器中，导致游离酶示踪剂被反应器捕获。捕获的酶示踪剂采用对碘酚-鲁米诺- h2o2化学发光体系检测。在最优条件下，甲胎蛋白浓度在5.0 ~ 100 ng/ml范围内，化学发光强度下降与甲胎蛋白浓度成正比，检测限为2.7 ng/ml，信噪比为3。该免疫传感器系统具有可接受的重复性和稳定性。用该方法测定了临床血清样本，结果与免疫放射测定法的结果一致。(c) 2006 Elsevier B.V.版权所有
A flow-injection chemiluminescent immunoassay system based on a novel transparent immunoaffinity reactor is proposed for the quantitation of alpha-fetoprotein. The reactor prepared with alpha-fetoprotein immobilized epoxysilane modified glass microbeads was used as an immunosensor for chemiluminescent detection. With a non-competitive immunoassay formal, the proposed immunosensor system is a low cost, flexible and rapid assay for alpha-fetoprotein. After an off-line incubation of the analyte alpha-fetoprotein with horseradish peroxidase-labeled alpha-fetoprotein antibody as enzyme tracer, the mixture was injected into the reactor, which led to trapping of the free enzyme tracer by the reactor. The trapped enzyme tracer was detected by the p-iodophenol-luminol-H2O2 chemiluminescence system. Under optimal conditions, the decrease in chemiluminescence intensity was proportional to the alpha-fetoprotein concentration in the range of 5.0-100 ng/ml with a detection limit of 2.7 ng/ml at a signal/noise ratio of 3. The immunosensor system showed an acceptable reproducibility and stability. Clinical serum samples were assayed with this method and the results were in acceptable agreement with those obtained from immunoradiometric assay. (c) 2006 Elsevier B.V. All rights reserved.