Characterization of a mouse ctgf 3'-UTR segment that mediates repressive regulation of gene expression.
Characterization of a mouse ctgf 3'-UTR segment that mediates repressive regulation of gene expression.
复制标题
介导基因表达抑制性调节的小鼠 ctgf 3-UTR 片段的表征。
DOI：
10.1006/bbrc.2000.3780
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发表时间：
2000
影响因子：
3.1
通讯作者：
Masaharu Takigawa
中科院分区：
文献类型：
--
作者：
Seiji Kondo;S. Kubota;Takanori Eguchi;T. Hattori;Tohru Nakanishi;Toshio Sugahara;Masaharu Takigawa
我们分离了小鼠结缔组织生长因子（ctgf/fisp 12）基因的一小段3 '-非翻译区（3'-UTR）并对其功能进行了评价。比较人和小鼠ctgf 3 '-UTR的核苷酸序列，发现一个91个碱基的保守小片段。3 '-UTR的相应片段的同源性高达82.4%，而3'-UTR之间的总同源性为71.8%。为研究ctgf基因保守片段的功能，从NIH 3 T3细胞中扩增了小鼠ctgf cDNA的相应序列。当它与标记基因融合后，对基因表达有明显的抑制作用。正义形式的抑制作用比反义形式更显著。对这些序列的计算机分析预测了稳定的二级结构，表明它们在RNA水平上起作用。有义和反义形式的预测结构似乎略有不同，这与抑制功能的差异一致。这些发现确定了小鼠ctgf基因中的保守小元件作为基因表达的有效负调控因子，其可能在转录后水平起作用。
We isolated a small segment of the 3'-untranslated region (3'-UTR) in the mouse connective tissue growth factor (ctgf/fisp12) gene and evaluated its functionality. Comparison of the nucleotide sequences of human and mouse ctgf 3'-UTRs revealed a conserved small segment of 91 bases. The corresponding segments of the 3'-UTRs shared as much as 82.4% homology, whereas the overall homology between the 3'-UTRs was 71.8%. To study the functionality of the conserved segment, the corresponding region of mouse ctgf cDNA was amplified from NIH3T3 cells. When it was fused downstream of a marker gene, it showed remarkable repressive effects on gene expression. The repressive effect of the sense form was more prominent than that of the antisense form. Computer analyses of these sequence predicted stable secondary structures, suggesting that they act at the RNA level. The predicted structures of the sense and antisense forms appeared to be slightly different, which is consistent with the difference in repressive function. These findings defined the conserved small element in the mouse ctgf gene as a potent negative regulator of gene expression, which may act at a posttranscriptional level.
影响因子：
20.3
作者：
D. Brigstock
通讯作者：
D. Brigstock
影响因子：
3.3
作者：
IGARASHI, A;OKOCHI, H;GROTENDORST, GR
通讯作者：
GROTENDORST, GR
影响因子：
3.3
作者：
Claffey, KP;Shih, SC;Detmar, M
通讯作者：
Detmar, M