PROFILES OF ENZYMES OF SUCROSE METABOLISM IN THE LEAVES OF CHICORY (CICHORIUM-INTYBUS) DURING DEVELOPMENT
PROFILES OF ENZYMES OF SUCROSE METABOLISM IN THE LEAVES OF CHICORY (CICHORIUM-INTYBUS) DURING DEVELOPMENT
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DOI：
10.1016/0168-9452(91)90088-p
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发表时间：
1991-01-01
期刊：
影响因子：
5.2
通讯作者：
SINGH, R
中科院分区：
文献类型：
--
作者：
GUPTA, AK;MANN, P;SINGH, R
在菊苣发育的各个阶段，蔗糖磷酸合酶（SPS）活性均匀分布在叶片和叶柄之间。 与蔗糖合酶（SS）相比，在生长初期（2月）和中期（4月）叶片和叶柄中SPS的活性较高。 这些酶在发育中期的叶片和叶柄中的活性比早期和晚期（7月）高3-10倍，并且与果聚糖合成和根（库）中干重积累的最大速率相一致。 从(CO2)-C-14的固定和运输来看，此阶段向根部供应的光合产物比后期要多得多。 看来，在发育中期，除了SPS活性之外，叶子还利用增强的SS活性来满足库区的光合产物需求。 酸性蔗糖酶在生长过程中仍然是蔗糖酶的主要形式。 在早期和中期，叶柄显示出两种酸性转化酶，而叶片在后期除了两种酸性转化酶外还含有一种额外的低分子量碱性转化酶。 开花时直立茎的叶子仅含有一种形式的酸性转化酶。
The activity of sucrose phosphate synthase (SPS) was evenly distributed between the lamina and petiole at all stages of development of Cichorium intybus. As compared to sucrose synthase (SS), the activities of SPS in lamina and petiole at both the early (February) and mid-stages (April) of growth were higher. Activities of these enzymes in the lamina and petiole at the mid-stage of development were 3-10 times higher as compared to the early and late (July) stages and coincided with the maximum rate of fructan synthesis and dry weight accumulation in roots (sink). The supply of photosynthates to the roots, as revealed by (CO2)-C-14 fixation and its transportation at this stage, was much more when compared to that at late stage. It appeared that at mid-development leaves made use of enhanced activity of SS in addition to SPS activity for meeting the photosynthate requirement of the sink region. Acid invertase remained the major form of invertase during growth. At the early and mid-stages, petioles showed two acid invertases whereas lamina, in addition to two acid invertases, contained an additional low molecular weight alkaline invertase at the late stage. Leaves of the erect stem at the time of flowering contained only one form of acid invertase.