Comparative genomic analysis of bacteriophage EP23 infecting Shigella sonnei and Escherichia coli
Comparative genomic analysis of bacteriophage EP23 infecting Shigella sonnei and Escherichia coli
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DOI：
10.1007/s12275-011-1577-0
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发表时间：
2011-12-01
影响因子：
3
通讯作者：
Kim, Kyoung-Ho
中科院分区：
文献类型：
--
作者：
Chang, Ho-Won;Kim, Kyoung-Ho
分离并鉴定了感染大肠杆菌和宋内志贺氏菌的噬菌体 EP23。噬菌体形态与噬菌体科成员相似。使用 454 焦磷酸测序对 44,077 bp 基因组进行了完整测序。比较基因组和系统发育分析表明，EP23 与感染 Sodalis Glossinidius 的噬菌体 SO-1 和感染工程大肠杆菌的噬菌体 SSL-2009a 关系最密切。基因组比较表明，EP23和SO-1尽管宿主在属水平上分离，但在基因顺序和氨基酸相似性方面非常相似。 EP23和SSL-2009a在它们的基因之间显示出高度的氨基酸相似性，但有证据表明SSL-2009a中存在多个重组事件。比较基因组分析的结果进一步了解了 EP23 及其噬菌体亲属之间的进化和关系。
Bacteriophage EP23 that infects Escherichia coli and Shigella sonnei was isolated and characterized. The bacteriophage morphology was similar to members of the family Siphoviridae. The 44,077 bp genome was fully sequenced using 454 pyrosequencing. Comparative genomic and phylogenetic analyses showed that EP23 was most closely related to phage SO-1, which infects Sodalis glossinidius and phage SSL-2009a, which infects engineered E. coli. Genomic comparison indicated that EP23 and SO-1 were very similar with each other in terms of gene order and amino acid similarity, even though their hosts were separated in the level of genus. EP23 and SSL-2009a displayed high amino acid similarity between their genes, but there was evidence of several recombination events in SSL-2009a. The results of the comparative genomic analyses further the understanding of the evolution and relationship between EP23 and its bacteriophage relatives.