The amelioration of cardiac dysfunction after myocardial infarction by the injection of keratin biomaterials derived from human hair
The amelioration of cardiac dysfunction after myocardial infarction by the injection of keratin biomaterials derived from human hair
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DOI：
10.1016/j.biomaterials.2011.08.057
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发表时间：
2011-12-01
期刊：
影响因子：
14
通讯作者：
Zhang, Jinying
中科院分区：
文献类型：
--
作者：
Shen, Deliang;Wang, Xiaofang;Zhang, Jinying
急性心肌梗死后心功能不全是晚期心肌病的主要原因。常规的药物治疗依赖于迅速再灌注和预防重复的适应不良通路。角蛋白生物材料可以以自体方式制造，并且在各种组织再生模型中有效。然而，其在心脏再生中的潜在应用尚未得到测试。从人发中提取角蛋白生物材料，并对其结构形态、有益因子的携带、与心肌细胞的生物相容性以及体内降解特性进行了表征。在递送到梗死的大鼠心脏后，角蛋白支架被心肌细胞和内皮细胞有效地浸润。注射角蛋白生物材料促进血管生成，但不会加剧MI后心脏的炎症。与对照注射的动物相比，角蛋白生物材料注射的动物在随后的8周内表现出心脏功能的保护和不利的心室重构的减弱。组织蛋白质印迹分析揭示了在角蛋白注射的心脏中有益因子（BMP 4、NGF、TGF-β）的上调。这种生物材料的显著功能益处、制造简单性和潜在的自体性质为进一步转化为临床提供了动力。(C)2011爱思唯尔有限公司保留所有权利。
Cardiac dysfunction following acute myocardial infarction is a major cause of advanced cardiomyopathy. Conventional pharmacological therapies rely on prompt reperfusion and prevention of repetitive maladaptive pathways. Keratin biomaterials can be manufactured in an autologous fashion and are effective in various models of tissue regeneration. However, its potential application in cardiac regeneration has not been tested. Keratin biomaterials were derived from human hair and its structure morphology, carryover of beneficial factors, biocompatibility with cardiomyocytes, and in vivo degradation profile were characterized. After delivery into infarcted rat hearts, the keratin scaffolds were efficiently infiltrated by cardiomyocytes and endothelial cells. Injection of keratin biomaterials promotes angiogenesis but does not exacerbate inflammation in the post-MI hearts. Compared to control-injected animals, keratin biomaterials-injected animals exhibited preservation of cardiac function and attenuation of adverse ventricular remodeling over the 8 week following time course. Tissue western blot analysis revealed up-regulation of beneficial factors (BMP4, NGF, TGF-beta) in the keratin-injected hearts. The salient functional benefits, the simplicity of manufacturing and the potentially autologous nature of this biomaterial provide impetus for further translation to the clinic. (C) 2011 Elsevier Ltd. All rights reserved.