Can mesenchymal stem cells pretreated with platelet-rich plasma modulate tissue remodeling in a rat with burned skin?
Can mesenchymal stem cells pretreated with platelet-rich plasma modulate tissue remodeling in a rat with burned skin?
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DOI：
10.1139/bcb-2016-0224
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发表时间：
2017-01-01
影响因子：
2.9
通讯作者：
Abusree, Azza
中科院分区：
文献类型：
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作者：
Ahmed, Hanan Hosni;Rashed, Laila Ahmed;Abusree, Azza
我们的目的是研究富血小板血浆（PRP）对骨髓间充质干细胞（BM-MSC）增殖的影响，并探讨其在实验性烧伤愈合中的作用及其可能的作用机制。我们的工作分为体外和体内研究。体外研究包括未经处理的 MSC 和经过 PRP 处理的 MSC。评估TGF-β水平和细胞增殖。在体内研究中，72只大鼠平均分为6组：对照组、烧伤组、MSCs烧伤组、PRP烧伤组、MSCs和PRP两者烧伤组、MSCs经PRP预处理烧伤组。伤后第7天、第20天,采用ELISA法测定血清转化生长因子β(TGF-β)、肿瘤坏死因子α(TNF-β)的含量以及皮肤组织中白细胞介素-10(IL-10)的含量;通过实时PCR对所有组进行组织病理学和MMP-1、TIMP-2、Ang-1、Ang-2和vimentin的基因表达。体外：与对照组相比，PRP 治疗组的 MSC 和 TGF-β 增殖增加。体内：Ang-1、Ang-2和波形蛋白上调，而MMP-1和TIMP-2下调。所有治疗组中 TGF-β 和 IL-10 均升高，而 TNF-α 均降低，其中在第 20 天的 MSC 和 PRP 中更为显着。所有治疗组中烧伤皮肤的组织病理学均得到改善，特别是在烧伤后 20 天用 PRP 预处理的 MSC 中。
Our aim was to study the effect of platelet-rich plasma (PRP) on the proliferation of bone-marrow-derived mesenchymal stem cells (BM-MSCs) and to investigate their roles in the healing of experimental burn injury and the possible mechanism of action. Our work was divided into in-vitro and in-vivo studies. The in-vitro study included untreated MSCs and MSCs treated with PRP. Levels of TGF-beta and cell proliferation were assessed. In the in-vivo study, 72 rats were distributed equally among 6 groups: control, burn, burn with MSCs, burn with PRP, burn with both MSCs and PRP, and burn with MSCs pretreated with PRP. On the 7th and 20th day after injury, the serum levels of transforming growth factor beta (TGF-beta) and tumor necrosis factor alpha (TNF-beta), as well as interleukin-10 (IL-10) levels in skin tissue were measured by ELISA; histopathology and gene expression of MMP-1, TIMP-2, Ang-1, Ang-2, and vimentin by real-time PCR were performed in all groups. In vitro: proliferation of MSCs and TGF-beta increased in the PRP-treated group compared with the control group. In vivo: Ang-1, Ang-2, and vimentin were upregulated, whereas MMP-1 and TIMP-2 were downregulated. TGF-beta and IL-10 were increased, whereas TNF-alpha was decreased in all treated groups with more significance in MSCs and PRP on day 20. Histopathology of burn skin was improved in all treated groups, particularly in MSCs pretreated with PRP 20 days post-burn.