Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice
Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice
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6-磷酸海藻糖合酶基因 OsTPS1 的过度表达增强了水稻的非生物胁迫耐受性
DOI：
10.1007/s00425-011-1458-0
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发表时间：
2011-11-01
期刊：
影响因子：
4.3
通讯作者：
Wang, Xi-Ping
中科院分区：
文献类型：
--
作者：
Li, Hao-Wen;Zang, Bai-Sheng;Wang, Xi-Ping
海藻糖在多种生物的代谢调节和非生物胁迫耐受中起着重要作用。在植物中，它的生物合成由两个关键酶催化：海藻糖-6-磷酸合成酶（TPS）和海藻糖-6-磷酸磷酸酶（TPP）。水稻（Oryza sativa）基因组中含有11个OsTPS基因，其中只有OsTPS 1具有TPS活性。为了证明OsTPS 1的生理功能，我们将其导入水稻，发现OsTPS 1过表达提高了水稻幼苗对冷、高盐和干旱处理的耐受性，而没有其他显著的表型变化。在过表达OsTPS 1的转基因系中，海藻糖和脯氨酸浓度高于野生型，并且一些应激相关基因上调，包括WSI 18、RAB 16 C、HSP 70和ELIP。这些结果表明OsTPS 1可能通过增加海藻糖和脯氨酸的含量以及调节胁迫相关基因的表达来增强植物的非生物胁迫耐受性。此外，我们发现，一些II类TPS的过表达也增强了植物对非生物胁迫的耐受性。本研究将有助于阐明海藻糖代谢在高等植物非生物胁迫反应中的作用。
Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. In plants, its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity. To demonstrate the physiological function of OsTPS1, we introduced it into rice and found that OsTPS1 overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant phenotypic changes. In transgenic lines overexpressing OsTPS1, trehalose and proline concentrations were higher than in the wild type and some stress-related genes were up-regulated, including WSI18, RAB16C, HSP70, and ELIP. These results demonstrate that OsTPS1 may enhance the abiotic stress tolerance of plants by increasing the amount of trehalose and proline, and regulating the expression of stress-related genes. Furthermore, we found that overexpression of some Class II TPSs also enhanced plant tolerance of abiotic stress. This work will help to clarify the role of trehalose metabolism in abiotic stress response in higher plants.