Humidity governs the wall-inhabiting fungal community composition in a 1600-year tomb of Emperor Yang
Humidity governs the wall-inhabiting fungal community composition in a 1600-year tomb of Emperor Yang
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湿度控制着1600年前炀帝陵墓壁上的真菌群落组成
DOI：
10.1038/s41598-020-65478-z
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发表时间：
2020-05-21
影响因子：
4.6
通讯作者：
Shen, Yang
中科院分区：
文献类型：
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作者：
Li, Yonghui;Huang, Zhi;Shen, Yang
丝状真菌引起的生物退化通常对建筑遗产（如坟墓和历史遗址）构成威胁。为了明确了解微生物在墓葬中的变质现象，以及这些微生物是如何在各种环境因素的影响下形成的，我们利用变性梯度凝胶电泳（DGGE）指纹图谱技术，在不同高度调查了中国皇帝杨氏（公元前569-618）墓室中的真菌群落。相关的环境条件，如湿度、温度、高度和光照，也被评估。结果表明，杨帝墓室真菌种类多样，包括冬虫夏草、镰刀菌、石壶菌、Emericellopsis、Volutella、Cladosporium、Stachybotrys、Trichoderma、Cochlonema和2种未知真菌。优势菌种为葡萄霉属、镰刀菌属、木霉属和褐霉属。冗余分析（RDA）表明，湿度、温度、高度和光照对真菌群落的影响最为显著。湿度的变异程度最大（19.2%），其次是光照（18.3%）和高度（12.8%）。真菌丰富度和多样性指数与湿度呈显著正相关
Biodeterioration caused by filamentous fungi is often a threat to the architectural heritage (i.e. tombs and historic sites). To specifically understand the deterioration phenomena caused by microorganisms in tombs and how these are shaped due to various environmental factors, the fungal communities in the coffin chamber of the Chinese emperor Yang (BC 569-618) were investigated at different heights using denaturant gradient gel electrophoresis (DGGE) fingerprinting. The associated environmental conditions, such as humidity, temperature, height and illumination, were also assessed. The results showed that a great diversity of fungal species (Cordyceps, Fusarium, Harpochytrium, Emericellopsis, Volutella, Cladosporium, Stachybotrys, Trichoderma, Cochlonema and two unknown fungal species) was present in emperor Yang's coffin chamber. The predominant species were Stachybotrys, Fusarium, Trichoderma and Cochlonema. Redundancy analysis (RDA) indicated that humidity, temperature, height and illumination were the most significantly related factors shaping the fungal communities. Humidity showed the highest degree of variance description (19.2%) than all other environmental factors, followed by illumination (18.3%) and height (12.8%). Furthermore, fungal richness and diversity indices showed a positive correlation with humidity (p