The haemocytic origin of lymphoid organ spheroid cells in the penaeid prawn Penaeus monodon
The haemocytic origin of lymphoid organ spheroid cells in the penaeid prawn Penaeus monodon
复制标题
DOI：
10.3354/dao040085
复制
发表时间：
2000-03-14
影响因子：
1.4
通讯作者：
Owens, L
中科院分区：
文献类型：
--
作者：
Anggraeni, MS;Owens, L
对斑节对虾淋巴器官球状体细胞进行了研究。酚氧化酶和过氧化物酶测定表明，LOS细胞具有类似于半颗粒，特别是大颗粒血细胞的特点。酚氧化酶和过氧化物酶阳性LOS细胞的平均百分比分别为85 +/-23%和82 +/-23%。LOS细胞内酚氧化酶和过氧化物酶活性位点间无显著性差异（t = 1.617，df = 29，p > 0.05）。实验室对虾和养殖对虾的LOS细胞相对于基质细胞的相对截面积与对虾体重和体长的增长均不相关（p > 0.05）。凋亡检测结果显示，LOS细胞常发生凋亡，而基质细胞则不发生凋亡。实验室对虾（52 +/- 24%）和养殖对虾（80 +/- 12%）的凋亡球状体细胞的平均百分比存在显著差异（t =-5.533，df = 58，p < 0.05）。总之，LOS细胞具有吞噬外来物质，特别是病毒的外吞，颗粒血细胞的特点，并可能构成一个主要的机制，为Penaeid抗病毒防御。
Studies on lymphoid organ spheroid (LOS) cells of Penaeus monodon were undertaken. Phenoloxidase and peroxidase assays showed that LOS cells have characteristics similar to semi-granular and, in particular, large granular haemocytes. The mean percentage of LOS cells positive for phenoloxidase and peroxidase was 85 +/- 23 and 82 +/- 23 %, respectively. There was no significant difference between the sites of phenoloxidase and peroxidase activity in LOS cells (t = 1.617, df = 29, p > 0.05). The relative sectional area occupied by LOS cells relative to that of the stromal matrix cells from both laboratory-held and farmed prawns was not correlated to increasing weight or total length of the prawns (p > 0.05). An apoptosis detection assay showed that LOS cells were often apoptotic whilst stromal matrix cells were not. There was a significant difference (t = -5.533, df = 58, p < 0.05) in the mean percentage of apoptotic spheroid cells between laboratory-held prawns (52 +/- 24%) and farmed prawns with midcrop mortality syndrome (MCMS) (80 +/- 12%). In conclusion, LOS cells have the characteristics of exocytosed, granular haemocytes that have phagocytosed foreign material, particularly viruses, and probably constitute a major mechanism for penaeid antiviral defense.