The balance between endotoxin accumulation and clearance during particle-induced osteolysis in murine calvaria
The balance between endotoxin accumulation and clearance during particle-induced osteolysis in murine calvaria
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DOI：
10.1002/jor.20289
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发表时间：
2007-03-01
影响因子：
2.8
通讯作者：
Greenfield, Edward M.
中科院分区：
文献类型：
--
作者：
Tatro, Joscelyn M.;Taki, Naoya;Greenfield, Edward M.
即使没有临床或微生物感染证据，细菌内毒素也可能导致骨科植入物无菌松动。无菌松动过程中内毒素的一种潜在来源是全身循环的内毒素，源自肠道菌群、轻微感染或牙科手术，可能与磨损颗粒结合。目前的研究表明，当“无内毒素”的钛和聚乙烯颗粒植入小鼠颅骨时，系统产生的内毒素会积累。时程实验和使用无菌小鼠的实验排除了观察到的内毒素积累可能是由于细菌污染造成的可能性。相反，内毒素从最初携带大量粘附内毒素的钛颗粒中被清除。内毒素清除机制不依赖于呼吸爆发的诱导。综上所述，这些结果表明，内毒素积累和内毒素清除之间的平衡控制着植入在小鼠颅骨上的骨科磨损颗粒周围的内毒素的稳态水平。这种平衡可以调节小鼠颅骨模型以及无菌性松动患者的骨溶解率。 (c) 2006 年骨科研究学会。由 Wiley 期刊公司出版
Bacterial endotoxin may contribute to aseptic loosening of orthopedic implants even in the absence of clinical or microbiological evidence of infection. One potential source of endotoxin during aseptic loosening is systemically circulating endotoxin, derived from intestinal flora, minor infections, or dental procedures, that may bind to wear particles. The current study demonstrates that systemically derived endotoxin accumulates when 'endotoxin-free' titanium and polyethylene particles are implanted on murine calvaria. Time-course experiments and experiments using germfree mice rule out the possibility that the observed endotoxin accumulation may be due to bacterial contamination. In contrast, endotoxin is cleared from titanium particles that originally carry high amounts of adherent endotoxin. The mechanism of endotoxin clearance is not dependent on induction of a respiratory burst. Taken together, these results indicate that a balance between endotoxin accumulation and endotoxin clearance controls the steady-state level of endotoxin surrounding orthopedic wear particles implanted on murine calvaria. This balance may regulate the rate of osteolysis in the murine calvaria model as well as in patients with aseptic loosening. (c) 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.