Fast Detection of Volatile Organic Compounds from Bacterial Cultures by Secondary Electrospray Ionization-Mass Spectrometry
Fast Detection of Volatile Organic Compounds from Bacterial Cultures by Secondary Electrospray Ionization-Mass Spectrometry
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DOI：
10.1128/jcm.00392-10
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发表时间：
2010-12-01
影响因子：
9.4
通讯作者：
Hill, Jane E.
中科院分区：
文献类型：
--
作者：
Zhu, Jiangjiang;Bean, Heather D.;Hill, Jane E.
我们提出了一种新的应用二次电喷雾电离质谱（SESI-MS）作为细菌感染的实时临床诊断工具。已知由细菌作为代谢物以不同组合和量产生的挥发性有机化合物（VOC）产生某些细菌的特征气味。这些挥发性有机化合物包括一个特定的代谢谱，可用于物种或血清型鉴定，但快速和灵敏的分析方法是广泛的用途所必需的。在这项研究中，挥发性有机化合物的配置文件的五个细菌群从四个属，铜绿假单胞菌，金黄色葡萄球菌，大肠杆菌，沙门氏菌肠血清型鼠伤寒沙门氏菌，沙门氏菌肠血清型鸡白痢，其特征在于SESI-MS。13种化合物被确定从这些细菌培养物，这些挥发性有机化合物的组合创建了一个独特的模式，为每个属。此外，主成分分析（PCA）被应用于种或血清型的歧视。前三个主成分表现出这五个细菌群的代谢挥发性谱之间的明显分离，这与生长培养基无关。作为解决临床应用复杂性的第一步，进行了混合培养物的体外试验。结果表明，在混合培养物中的个别物种或血清型是可识别的生物VOC背景中，和检测到的挥发物的比率反映了混合物中的每种细菌的比例。我们的数据证实了SESI-MS在体外细菌种类或血清型的实时鉴定中的实用性，这在未来可能在诊断感染中发挥有前途的临床作用。
We propose a novel application of secondary electrospray ionization-mass spectrometry (SESI-MS) as a real-time clinical diagnostic tool for bacterial infection. It is known that volatile organic compounds (VOCs), produced in different combinations and quantities by bacteria as metabolites, generate characteristic odors for certain bacteria. These VOCs comprise a specific metabolic profile that can be used for species or serovar identification, but rapid and sensitive analytical methods are required for broad utility. In this study, the VOC profiles of five bacterial groups from four genera, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Salmonella enterica serovar Typhimurium, and Salmonella enterica serovar Pullorum, were characterized by SESI-MS. Thirteen compounds were identified from these bacterial cultures, and the combination of these VOCs creates a unique pattern for each genus. In addition, principal component analysis (PCA) was applied for the purpose of species or serovar discrimination. The first three principal components exhibit a clear separation between the metabolic volatile profiles of these five bacterial groups that is independent of the growth medium. As a first step toward addressing the complexity of clinical application, in vitro tests for mixed cultures were conducted. The results show that individual species or serovars in a mixed culture are identifiable among a biological VOC background, and the ratios of the detected volatiles reflect the proportion of each bacterium in the mixture. Our data confirm the utility of SESI-MS in real-time identification of bacterial species or serovars in vitro, which, in the future, may play a promising clinical role in diagnosing infections.