Redox changes accompanying the degradation of seed storage proteins in germinating rice.

Redox changes accompanying the degradation of seed storage proteins in germinating rice.
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DOI:
10.1093/pcp/pce119
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发表时间:
2001-08
影响因子:
4.9
通讯作者:
H. Yano;J. Wong;M. Cho;B. Buchanan
H. Yano;J. Wong;M. Cho;B. Buchanan
中科院分区:
生物学2区
文献类型:
--
作者:
H. Yano;J. Wong;M. Cho;B. Buchanan

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水稻种子萌发过程中储存蛋白(谷氨酸)的动员伴随着蛋白水解和二硫基团还原的有序顺序组合。在对完整种子中蛋白质的巯基进行单溴烷标记后,进行动员,然后应用非还原/还原二维page。
The mobilization of storage proteins (glutelins) in germinating rice seeds was accompanied by an ordered sequential combination of proteolysis and reduction of disulfide groups. Mobilization was followed by application of non-reducing/reducing two dimensional-PAGE after monobromobimane labeling of the sulfhydryl groups of the proteins in intact seeds.