Mapping and cloning recombinant breakpoints demarcating the hybrid sterility 6-specific sperm tail assembly defect.
Mapping and cloning recombinant breakpoints demarcating the hybrid sterility 6-specific sperm tail assembly defect.
复制标题
标定杂交不育 6 特异性精子尾部组装缺陷的重组断点的定位和克隆。
DOI：
10.1007/s003359900950
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Pilder,SH
中科院分区：
文献类型：
--
作者：
Samant,SA;Fossella,J;Silver,LM;Pilder,SH
Variants of the mousetcomplex known asthaplotypes(t)express factors that perturb sperm differentiation, resulting in the non-Mendelian transmission oftfrom +/theterozygous males and the sterility oft/thomozygous males. Previous studies of mice carrying heterospecific combinations of thetcomplex have revealed a 1-cM candidate locus,Hst6,for the distal-most of these factors,Tcd/Tcs2.Males heterozygous for theM. spretusallele ofHst6and athaplotype (Hst6s/t) are sterile, expressing an abnormality in sperm flagellar curvature (``curlicue'') indistinguishable from one exhibited by sperm fromt/thomozygotes.Hst6s/Hst6smales are also sterile; however, sperm produced by these males are completely immotile owing to the absence of assembled flagella. Recent studies have shown that the complete presentation of ``curlicue'' derives from expression of at least two factors within the locus, Curlicue a (Ccua) proximally and Curlicue b (Ccub) distally, with a factor affecting sperm-oolemma penetration,Stop1p,mapping between them. In the present report, we have examined expression of theHst6-specific flagellar assembly phenotype in sperm from mice homozygous forM. spretus–M. m. domesticusrecombinant Chr 17 homologs whose breakpoints map within theHst6locus. SSLP analysis of these homologs has demonstrated that the flagellar assembly defect maps to less than 0.2 cM betweenD17Mit61andD17Mit135,coincident withCcua.SSR content analysis of 23 BACs mapping to four contigs within theHst6locus has resulted in isolation of proximal and distal recombinant breakpoints circumscribing the flagellar assembly phenotype/Ccuafactor. In addition, we have provided increased high-resolution mapping of theStop1pandCcubfactors. These new data enhance our ability to isolate and characterize candidates forTcd/Tcs2.
