Zebrafish as an Emerging Model Organism to Study Angiogenesis in Development and Regeneration.
Zebrafish as an Emerging Model Organism to Study Angiogenesis in Development and Regeneration.
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DOI：
10.3389/fphys.2016.00056
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发表时间：
2016
影响因子：
4
通讯作者：
Egaña JT
中科院分区：
文献类型：
--
作者：
Chávez MN;Aedo G;Fierro FA;Allende ML;Egaña JT
血管生成是新血管从先前存在的血管形成的过程，并且在包括胚胎发育、组织修复和疾病在内的几种情况中起关键作用。此外，增强的治疗性血管生成是再生医学领域的主要目标，并且人工组织和器官的有效血管化是实施组织工程方法的主要障碍之一，而另一方面，抑制血管生成是抑制例如肿瘤生长的关键治疗靶标。在过去的几十年里，对这一过程中所涉及的细胞和分子机制的理解一直是深入研究的问题。在这方面，已经建立了几种体外和体内模型来可视化和研究内皮祖细胞的迁移、内皮小管的形成和新血管网络的产生，同时评估促进或抑制这些过程的条件和治疗。在这篇综述中，我们地址和比较最常用的实验模型，研究血管生成在体外和体内。特别是，我们专注于实施斑马鱼（Danio rerio）作为一个模型来研究血管生成和讨论的优点，尚未探讨其作为模式生物的可能性。
Angiogenesis is the process through which new blood vessels are formed from preexisting ones and plays a critical role in several conditions including embryonic development, tissue repair and disease. Moreover, enhanced therapeutic angiogenesis is a major goal in the field of regenerative medicine and efficient vascularization of artificial tissues and organs is one of the main hindrances in the implementation of tissue engineering approaches, while, on the other hand, inhibition of angiogenesis is a key therapeutic target to inhibit for instance tumor growth. During the last decades, the understanding of cellular and molecular mechanisms involved in this process has been matter of intense research. In this regard, several in vitro and in vivo models have been established to visualize and study migration of endothelial progenitor cells, formation of endothelial tubules and the generation of new vascular networks, while assessing the conditions and treatments that either promote or inhibit such processes. In this review, we address and compare the most commonly used experimental models to study angiogenesis in vitro and in vivo. In particular, we focus on the implementation of the zebrafish (Danio rerio) as a model to study angiogenesis and discuss the advantages and not yet explored possibilities of its use as model organism.