Antitumor effects of saponin extract from Patrinia villosa (Thunb.) Juss on mice bearing U14 cervical cancer
Antitumor effects of saponin extract from Patrinia villosa (Thunb.) Juss on mice bearing U14 cervical cancer
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DOI：
10.1002/ptr.2354
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发表时间：
2008-05-01
影响因子：
7.2
通讯作者：
Zhou, Shaobo
中科院分区：
文献类型：
--
作者：
Zhang, Tao;Li, Qingwang;Zhou, Shaobo
从败酱草（SPVJ）中提取的皂苷是一种被传统医生广泛使用的中药。在这项研究中，我们在患有 U14 宫颈癌的小鼠中研究了 SPVJ 的抗肿瘤作用和可能的机制。结果显示，SPVJ（50 mg/kg和100 mg/kg）有效降低了U14宫颈肿瘤的重量（分别为35.1%和57.1%）。与对照组相比，SPVJ（100 mg/kg）显着增加G0/G1期肿瘤细胞（38.1% vs 68.5%），增加凋亡（9.4% vs 28.9%）和G0/G1期细胞数，减少S期（41% vs 26.2%）和G2/M（20.9% vs 5.3%）细胞数，抑制增殖细胞肿瘤细胞核抗原（PCNA）（80.6% vs 21.8%），突变型p53（66.4% vs 33.5%）和bcl-2蛋白（78.2% vs 20.3%）表达降低。 SPVJ抗肿瘤作用的机制可能与抑制G0/G1期肿瘤细胞，诱导细胞凋亡以及抑制PCNA、突变型P53和Bcl-2蛋白的表达有关。版权所有 (C) 2008 约翰·威利父子有限公司
Saponin extracted from Patrinia villosa (Thunb.) Juss (SPVJ) is a Chinese medicine which is used widely by traditional medicine doctors. In this study, the antitumor effects and the possible mechanisms of SPVJ were investigated in mice bearing U14 cervical cancer. The results showed that SPVJ (50 mg/kg and 100 mg/kg) effectively reduced the weight of U14 cervical tumor (35.1% and 57.1%, respectively). Compared with the control group, SPVJ (100 mg/kg) significantly increased tumor cells in the G0/G1 phase (38.1% vs 68.5%), increased the number of cells in apoptosis (9.4% vs 28.9%) and G0/G1 phase and decreased the number of cells in S phase (41% vs 26.2%) and G2/M (20.9% vs 5.3%), inhibited proliferating cell nuclear antigen (PCNA) of tumor cell (80.6% vs 21.8%), decreased the expression of mutant p53 (66.4% vs 33.5%) and bcl-2 protein (78.2% vs 20.3%). The mechanism of the SPVJ antitumor effect might be associated with inhibition of tumor cells in G0/G1 phase, inducing: apoptosis and inhibiting the expression of PCNA, mutant P53 and Bcl-2 protein. Copyright (C) 2008 John Wiley & Sons, Ltd.