CENTRAL IMPLANTS OF DILUTED ESTRADIOL - INDEPENDENT EFFECTS ON INGESTIVE AND REPRODUCTIVE BEHAVIORS OF OVARIECTOMIZED RATS
CENTRAL IMPLANTS OF DILUTED ESTRADIOL - INDEPENDENT EFFECTS ON INGESTIVE AND REPRODUCTIVE BEHAVIORS OF OVARIECTOMIZED RATS
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DOI：
10.1016/0006-8993(89)90062-0
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发表时间：
1989-07-10
期刊：
影响因子：
2.9
通讯作者：
BEIKIRCH, RJ
中科院分区：
文献类型：
--
作者：
BUTERA, PC;BEIKIRCH, RJ
目前的实验是为了评估雌激素对进食和性行为的影响在大脑中单独组织的假设。33只去卵巢大鼠植入双侧引导管，分别指向室旁核(PVN)、视前内侧区(MPOA)或下丘脑后部(PH)。接受PVN植入的受试者被未稀释的雌二醇、3：1或10：1的胆固醇和雌二醇混合物刺激。其他组的动物接受未稀释的雌二醇治疗。所有女性都受到胆固醇和雌二醇的刺激，总共产生了66个刺激点。组织学分析显示，与胆固醇植入物相比，PVN中未稀释的雌二醇减少了食物摄入量和体重。更重要的是，PVN中的稀释雌二醇植入物显著降低了食物摄入量和体重。相比之下，MPOA、PH或下丘脑腹内侧核(VMN)中的未稀释雌二醇对摄食或体重没有显著影响。方差分析表明，种植体位置对女性性行为有显著的主效应。Newman-Keuls测试表明，PVN中的稀释雌二醇植入物产生的前凸商数和质量分数明显低于VMH植入物。通过比较产生阴道细胞角化的植入物和不产生角化的植入物的结果，评估了观察到的行为变化是由于激素的外周效应而不是中枢效应的可能性。这些组之间在任何其他因变量上没有显著差异，表明外周雌二醇足以诱导阴道细胞角化既不是必要的，也不足以解释行为变化。这些发现清楚地表明，雌激素应用于特定的大脑区域可以对进食和性行为产生独立的影响。这些结果还表明，雌二醇对摄食量的影响是通过其在下丘脑室旁核的作用来实现的，而对生殖行为的影响则涉及雌二醇在下丘脑室旁核的作用。
The present experiment was undertaken to evaluate the hypothesis that the effects of estrogens on feeding and sexual behaviors are organized separately within the brain. Thirty-three ovariectomized rats were implanted with bilateral guide cannulae aimed at either the paraventricular nucleus (PVN), medical preoptic area (MPOA), or posterior hypothalamous (PH). Subjects that received PVN implants were stimulated with either undiluted estradiol, a 3:1, or 10:1 mixture of cholesterol and estradiol. Animals in the other groups were treated with undiluted estradiol. All females were stimulated with cholesterol and estradiol, yielding a total of 66 stimulation sites. Histological analysis revealed that, compared to cholesterol implants, undiluted estradiol in the PVN reduced food intake and body weight. More importantly, diluted estradiol implants in the PVN significantly lowered food intake and body weight. In contrast, undiluted estradiol in the MPOA, PH, or ventromedial hypothalamus (VMN) had no significant effects on feeding or body weight. Analyses of variance revealed significant main effects of implant location on female sexual behavior. Newman-Keuls tests indicated that diluted estradiol implants in the PVN produced lordosis quotients and quality scores that were significantly lower than those obtained with VMH implants. The possibility that the behavioral changes observed were due to peripheral rather than central effects of the hormone was evaluated by comparing the results of implants that produced vaginal cell cornification to those that did not. There were no significant differences between these groups on any of the other dependent variables, indicating that peripheral estradiol sufficient to induce vaginal cell cornification was neither necessary nor sufficient to account for the behavioral changes. These findings clearly indicate the estradiol applied to particular brain areas can produce independent effects on feeding and sexual behaviors. The results also suggest that the effects of estradiol on food intake are mediated by its actions in the PVN, whereas effects on reproductive behavior involve actions of estradiol in the VMH.