Enzymatic synthesis of anandamide, an endogenous cannabinoid receptor ligand, through N-acylphosphatidylethanolamine pathway in testis: involvement of Ca(2+)-dependent transacylase and phosphodiesterase activities.
Enzymatic synthesis of anandamide, an endogenous cannabinoid receptor ligand, through N-acylphosphatidylethanolamine pathway in testis: involvement of Ca(2+)-dependent transacylase and phosphodiesterase activities.
复制标题
通过睾丸中 N-酰基磷脂酰乙醇胺途径酶促合成 anandamide（一种内源性大麻素受体配体）：参与 Ca(2) 依赖性转酰基酶和磷酸二酯酶活性。
DOI：
--
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
K. Waku
中科院分区：
文献类型：
--
作者：
T. Sugiura;S. Kondo;A. Sukagawa;Takashi Tonegawa;S. Nakane;A. Yamashita;K. Waku
大鼠睾丸中含有大量的N-酰基乙醇胺，包括N-花生四烯酸乙醇胺（花生四烯酸乙醇胺）和N-酰基磷脂酰乙醇胺（N-酰基PE），包括N-花生四烯酸PE。两类的N-酰基部分的脂肪酸谱彼此相似。我们证实，睾丸微粒体含有磷酸二酯酶活性，催化花生四烯酸酯释放花生四烯酸酯。它们还含有催化花生四烯酸从二酰基磷脂的1-位转移至PE以形成N-花生四烯酸酰PE的酶活性。这些结果表明，N-酰基PE途径是重要的合成花生四烯酸在这个组织中。
Rat testis was shown to contain significant amounts of both N-acylethanolamine, including N-arachidonoylethanolamine (anandamide), and N-acylphosphatidylethanolamine (N-acylPE), including N-arachidonoylPE. The fatty acid profiles of the N-acyl moieties of the two classes resembled each other. We confirmed that testis microsomes contain a phosphodiesterase activity catalyzing the release of anandamide from N-arachidonoylPE. They also contain an enzyme activity catalyzing the transfer of arachidonic acid from the 1-position of diacylphospholipids to PE to form N-arachidonoylPE. These results suggest that the N-acylPE pathway is important in the synthesis of anandamide in this tissue.