ASSOCIATION OF INCREASED SPONTANEOUS MUTATION-RATES WITH HIGH-LEVELS OF TRANSCRIPTION IN YEAST
ASSOCIATION OF INCREASED SPONTANEOUS MUTATION-RATES WITH HIGH-LEVELS OF TRANSCRIPTION IN YEAST
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DOI：
10.1126/science.7777859
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发表时间：
1995-06-16
期刊：
影响因子：
56.9
通讯作者：
JINKSROBERTSON, S
中科院分区：
文献类型：
--
作者：
DATTA, A;JINKSROBERTSON, S
复杂的过程，如转录，复制，修复和重组需要染色质结构的变化和许多反式作用因子与DNA序列的相互作用，提高了这些过程可能是相互关联的可能性。在这里，研究了转录对酿酒酵母中自发突变率的影响。在高度诱导型启动子的控制下使用lys2移码等位基因，当突变基因被高度转录时，自发回复的速率显示出增加。因此，转录活性DNA和增强的自发突变率在酵母中是相关的。
Complex processes such as transcription, replication, repair, and recombination require changes in chromatin structure and the interactions of numerous trans-acting factors with DNA sequences, raising the possibility that these processes may be interrelated. Here the effect of transcription on the rate of spontaneous mutation in the yeast Saccharomyces cerevisiae was examined. With the use of a lys2 frameshift allele under the control of a highly inducible promoter, the rate of spontaneous reversion was shown to increase when the mutant gene was highly transcribed. Thus, transcriptionally active DNA and enhanced spontaneous mutation rates are associated in yeast.